This study was conducted to develop a rapid and accurate method for Aflatoxin B1 (AFB1) detection in peanut oil. For accelerating the reaction, AFB1 was enriched via AC electrokinetics, while the anti-AFB1 monoclonal antibody was immobilized onto a microchip which was acted as a sensitive component. Subsequently, the enriched AFB1 reacted with the antibodies, leading to the change of capacitance regularity. Based on the above principle, the AFB1 immunosensor was finally created to achieve high sensitive and quantitative analysis of AFB1 in edible peanut oil only within 30 s including sampling, incubating and testing. For this method, in test solution, linear range and relative standard deviation (n=5) were 10-6-10-2 μg/mL (R2=0.993 6) and 6.63%-15.60%, respectively. The limit of detection and limit of quantitation reached 2.01×10-7 μg/mL (3 σ/S) and 2.35×10-7 μg/mL (10 σ/S), respectively, with the average recovery of standard addition in peanut oil of 85.20%-97.7%. Besides, this method showed the anti-interference ability to other common toxins in food, such as AFB2, AFM1, AFG1, OTA, DON, ZEN, etc. In conclusion, this method is accurate, rapid, and highly sensitive to be used for the determination of trace amount AFB1 in peanut oil.
LIU Wei
,
ZHANG Jing
,
LI Shuhui
,
ZHANG Yuhao
,
LIU Xiaozhu
,
MA Liang
. Rapid and sensitive detection of Aflatoxin B1 in peanut oil by immunosensor based on AC electrokinetics[J]. Food and Fermentation Industries, 2019
, 45(22)
: 246
-252
.
DOI: 10.13995/j.cnki.11-1802/ts.021578
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