This study was conducted to develop a rapid and accurate method for Aflatoxin B1 (AFB1) detection in peanut oil. For accelerating the reaction, AFB1 was enriched via AC electrokinetics, while the anti-AFB1 monoclonal antibody was immobilized onto a microchip which was acted as a sensitive component. Subsequently, the enriched AFB1 reacted with the antibodies, leading to the change of capacitance regularity. Based on the above principle, the AFB1 immunosensor was finally created to achieve high sensitive and quantitative analysis of AFB1 in edible peanut oil only within 30 s including sampling, incubating and testing. For this method, in test solution, linear range and relative standard deviation (n=5) were 10-6-10-2 μg/mL (R2=0.993 6) and 6.63%-15.60%, respectively. The limit of detection and limit of quantitation reached 2.01×10-7 μg/mL (3 σ/S) and 2.35×10-7 μg/mL (10 σ/S), respectively, with the average recovery of standard addition in peanut oil of 85.20%-97.7%. Besides, this method showed the anti-interference ability to other common toxins in food, such as AFB2, AFM1, AFG1, OTA, DON, ZEN, etc. In conclusion, this method is accurate, rapid, and highly sensitive to be used for the determination of trace amount AFB1 in peanut oil.
LIU Wei
,
ZHANG Jing
,
LI Shuhui
,
ZHANG Yuhao
,
LIU Xiaozhu
,
MA Liang
. Rapid and sensitive detection of Aflatoxin B1 in peanut oil by immunosensor based on AC electrokinetics[J]. Food and Fermentation Industries, 2019
, 45(22)
: 246
-252
.
DOI: 10.13995/j.cnki.11-1802/ts.021578
[1] IARC W G O T. Some traditional herbal medicines, some mycotoxins, naphthalene and styrene[J]. IARC Monographs on the Evaluation of Carcinogenic Risks to Humans, 2002,82:1-556.
[2] 褚庆华,张艺兵,鲍蕾. 农产品中真菌毒素的检测分析[M]. 北京:化学工业出版社, 2006.
[3] DUARTE S C, PENA A, LINO C M. A review on ochratoxin A occurrence and effects of processing of cereal and cereal derived food products[J]. Food Microbiology, 2010,27(2):187-198.
[4] LIU J, SUN L, ZHANG J, et al. Aflatoxin B1, zearalenone and deoxynivalenol in feed ingredients and complete feed from central China[J]. Food Additives & Contaminants. Part B, Surveillance, 2016,9(2):91-97.
[5] GB 2761—2017 食品安全国家标准食品中真菌毒素限量[S].北京:中国标准出版社,2017.
[6] 常纪文. 从欧盟立法看动物福利法的独立性[J]. 环球法律评论, 2006(3):343-351.
[7] AZRI F A, SELAMAT J, SUKOR R. Electrochemical Immunosensor for the detection of aflatoxin B1 in palm kernel cake and feed samples[J]. Sensors:2017,17(12):2 776.
[8] 王嫦嫦,马良,刘微,等. 基于先进材料的适配体传感器在真菌毒素快速检测中的研究进展[J/OL]. 食品科学:1-9[2019-11-05].http://kns.cnki.net/kcms/detail/111.2206.TS.20190507.1830.040.html.
[9] YUGENDER GOUN K, CATANANTE G, HAYAT A, et al. Disposable and portable electrochemical aptasensor for label free detection of aflatoxin B1 in alcoholic beverages[J]. Sensors & Actuators: B. Chemical, 2016,235:466-473.
[10] 谭红霞,马良,郭婷,等. 玉米赤霉烯酮新型生物传感器检测技术研究进展[J]. 食品与发酵工业,2019,45(2):240-246.
[11] LI S C, CHEN J H, CAO H, et al. Amperometric biosensor for aflatoxin B1 based on aflatoxin-oxidase immobilized on multiwalled carbon nanotubes[J]. Food Control, 2011,22(1):43-49.
[12] WU J. Interactions of electrical fields with fluids:laboratory-on-a-chip applications[J]. IET Nanobiotechnology, 2008,2(1):14-27.
[13] LIU X, CHENG C, WU J, et al. A low cost and palm-size analyzer for rapid and sensitive protein detection by AC electrokinetics capacitive sensing[J]. Biosensors and Bioelectronics, 2017,90:83-90.
[14] CUI H, WU J, EDA S, et al. Rapid capacitive detection of femtomolar levels of bisphenol A using an aptamer-modified disposable microelectrode array[J]. Microchimica Acta, 2015,182(13):2 361-2 367.
[15] LIN X, CHENG C, TERRY P, et al. Rapid and sensitive detection of bisphenol a from serum matrix[J]. Biosensors and Bioelectronics, 2017,91:104-109.
[16] CUI H, CHENG C, WU J, et al. Rapid detection of progesterone by commercially available microelectrode chips, 2013[C]. Sensors,2013 IEEE.IEEE, 2013:1.4.
[17] 李姗姗.基于交流电场的生物分子快速检测及其试验研究[D]. 哈尔滨:哈尔滨工业大学, 2014.
[18] PELUSO P, WILSON D S, DO D, et al. Optimizing antibody immobilization strategies for the construction of protein microarrays[J]. Analytical Biochemistry, 2003,312(2):113-124.
[19] 江虹,张琴,吴征真,等.双波长共振瑞利散射法快速测定皮蛋中的Cd[J].食品与发酵工业,2017,43(6):263-267.
[20] WANG L, ZHU F, ZHU Y, et al. Rapid and visual detection of aflatoxin B1 in foodstuffs using aptamer/G-quadruplex DNAzyme probe with low background noise[J]. Food Chemistry, 2019,271:581-587.
[21] LI X, CAO L, ZHANG Y, et al. Fabrication and modeling of an ultrasensitive label free impedimetric immunosensor for aflatoxin B1 based on protein A self-assembly modified gold 3D nanotube electrode ensembles[J]. Electrochimica Acta, 2017,247:1 052-1 059.
[22] MA H, SUN J, ZHANG Y, et al. Label-free immunosensor based on one-step electrodeposition of chitosan-gold nanoparticles biocompatible film on Au microelectrode for determination of aflatoxin B1 in maize[J]. Biosensors and Bioelectronics, 2016,80:222-229.
[23] XIAO M, BAI X, LIU Y, et al. Simultaneous determination of trace Aflatoxin B1 and Ochratoxin A by aptamer-based microchip capillary electrophoresis in food samples[J]. Journal of Chromatography A, 2018,1 569:222-228.
