In order to promote the extracellular secretion of phycocyanin, the effects of temperature, inducers (IPTG and lactose) and medium additives (glycine, Triton X-100, sodium dodecyl sulfate and Tween-80) on the fluorescence intensity of extracellular β-subunit C-phycocyanin (CpcB) of Spirulina secreted by E. coli were investigated. The results showed that the extracellular medium exhibited an apparent CpcB-derived fluorescence after the recombinant expression for 24 h at 25℃. The fluorescence intensity gradually increased in a time-dependent manner. Temperature was the key factor affecting the extracellular secretion. Lactose induction could significantly increase the fluorescence intensity of the extracellular CpcB, which reached the highest, about 5.1 times higher than that of the control, with the lactose concentration of 10 g/L. Comparably, the addition of IPTG had no effect on the fluorescence intensity. The extracellular fluorescence intensity increased 6.3 times with the addition of 25 g/L glycine in the medium, and 2.5 times when 1.5% of φ(Tween-80) was added. The extracellular secretion caused by altering culture conditions and supplementing additives facilitates the heterologous production of fluorescent phycocyanin in E. coli.
ZHAO Baoguo
,
JIANG Sudan
,
WANG Changtian
,
WU Xianjun
,
LI Pingping
. Effects of culture conditions and additives on extracellular secretion of a recombinant fluorescent beta-subunit phycocyanin[J]. Food and Fermentation Industries, 2020
, 46(12)
: 193
-198
.
DOI: 10.13995/j.cnki.11-1802/ts.023565
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