A bacterial detection method based on the size changes of gold nanoparticles

  • XU Zhewei ,
  • CHEN Kun ,
  • HU Changying
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  • 1(Jinan University, Packaging Engineering Institute, Zhuhai 519070,China)
    2(Jinan University, Department of Food Science and Engineering, Guangzhou 510632,China)
    3(South China University of Technology, Advanced Institute for Soft Matter Science and Technology, Guangzhou 510640,China)

Received date: 2020-02-07

  Online published: 2020-06-17

Abstract

A broad-spectrum quantitative detection method of Gram-positive bacteria was realized based on the competitive binding of Gram-positive bacteria and gold nanoparticles (GNPs) to lysozyme (LZM). LZM and GNPs were incubated under the optimized condition of 15 min, pH 8.0 and room temperature. The Gram-positive bacteria were quantitatively detected by ultraviolet-visible (UV-Vis) spectrum and laser light scattering (DLS). Compared with the UV-Vis spectrum, DLS showed higher sensitivity to particle size changes, and the addition of 0.14 mg/mL LZM solution could be reduced from 40 μL in UV-Vis method to 20 μL in DLS method when the signal intensity reached an acceptable range. Therefore, the limit of quantitation reduced from 1.26×107 CFU/mL to 6.87×105 CFU/mL. Compared with the Gram-negative bacteria, both quantitative methods showed good selectivity for Gram-positive bacteria, and both methods could realize the quantitative bacterial detection in real sample with the average recoveries of 83.0%-87.0% with the relative standard deviation of 2.4%-3.1%. The DLS quantification also showed higher sensitivity. Compared with the UV-Vis method, DLS showed the superiority in this competitive binding detection system, which could improve the sensitivity of detection.

Cite this article

XU Zhewei , CHEN Kun , HU Changying . A bacterial detection method based on the size changes of gold nanoparticles[J]. Food and Fermentation Industries, 2020 , 46(10) : 227 -232 . DOI: 10.13995/j.cnki.11-1802/ts.023547

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