In order to improve the freeze-drying survival rate and industrial production efficiency of Bifidobacterium breve cell mass, the lyophilized survival rate of B. breve in different molecular weight of saccharide(alcohol) and proteins was systematically determined and the ratio and concentration of the wet bacterial mass and protectant were optimized. The results showed that raffinose and sorbitol had the best protective effect and their combination further increased the survival rate of lyophilization to about 55%. Addition of protein or small molecules such as ascorbic acid, glutathione, betaine and trace elements did not improve the lyophilization protection effect. Nevertheless, the optimal amount of protein improved the porosity of bacterial powder. When the wet bacterial mass mixed with the protective agents were at ratio of 1∶1.2 and the total dry matter fraction was 25%, the survival rate of B. breve was reached to 80% and the viable cells were up to (1.61±0.27)×1012 CFU/g.
JIANG Wenxin
,
CUI Shumao
,
MAO Bingyong
,
TANG Xin
,
ZHAO Jianxin
,
ZHANG Hao
,
CHEN Wei
. Optimization of lyoprotectants for high-density freeze-dryingBifidobacterium breve[J]. Food and Fermentation Industries, 2020
, 46(9)
: 31
-36
.
DOI: 10.13995/j.cnki.11-1802/ts.023657
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