The present study attempted to characterize the involvement of exogenous spermidine (Spd) in the maintenance of quality attributes in postharvest peaches, and further analyze the involved mechanism regarding the interrelationship between polyamines synthesis and ethylene release. The selected peaches were immersed in Spd solutions at different concentrations (0, 0.5, 1 and 2 mmol/L) for 10 min at 20 ℃ respectively, after which the peaches were stored at 20 ℃ for eight days. The quality and physiology parameters, contents of ethylene and polyamines and expression levels of relevant genes were measured at two-day intervals during storage. The results showed that the 1 mmol/L Spd treatment could more significantly decrease the decay incidence, maintain the higher TSS and TA contents as well as enhance the accumulations of total phenol, flavonoids and anthocyanins compared with the either Spd treatment at the concentration of 0.5 or 2 mmol/L. Meanwhile, the 1 mmol/L Spd treatment also remarkably delayed the increase on the MDA content, relative electric conductivity and level of reactive oxygen radicals (ROS) in peaches. In addition, the Spd treatment effectively up-regulated the transcription level of PpSAMDC, PpSPDS, PpADC and down-regulated the levels of PpACS1 and PpACO1 in peaches, contributing to the increase on the polyamines content and the decrease on the ethylene release and ratios of E/Spd and E/Spm over the storage period. These results indicated that reverse suppression of ethylene synthesis and its related genes could be attributed to the promoted expression of genes involved in polyamines metabolism and the polyamines accumulation, which finally delayed the process of membrane-lipid peroxidation and maintain the better quality in peaches.
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