Adenosylmethionine (SAM) plays a vital role in the organisms’ metabolic activity as an important intermediate metabolite and has been widely used in pharmaceutical industry involving the therapies for liver, nervous systems, osteoarthritis and so on. Thus, production of SAM by enzyme transformation process exhibits potential industrial prospects. Codon optimization of the adenosylmethionine synthase gene sam2 derived from S. cerevisiae BY4741 was performed, and the gene was ligated to pET-28a(+) and pET-3b(+), resulting in recombinant plasmids pET-28a(+)-sam2 and pET-3b(+)-sam2, respectively, and was heterologous expressed in E. coli BL21(DE3) and E. coli Rosseta (DE3) respectively. Results showed that the recombinant strain E. coli BL21/ pET28a(+)-sam2 exhibited higher enzymatic activity up to 0.184 U/mL. Thus, the recombinant strain E. coli BL21/ pET-28a(+)-sam2 was chosen for the optimization of fermentation condition to further improve the enzymatic activity. Under optimal conditions, the enzyme activity of the recombinant adenosylmethionine synthase reached 0.245 U/mL, which was 34% higher than that before optimization.
MAO Zhiyi
,
TAN Xinyuan
,
CAO Rong
,
ZHANG Xiaojuan
,
FU Jing
,
XU Jianguo
,
XU Guoqiang
,
ZHANG Xiaomei
,
XU Zhenghong
. Heterologous expression of adenosylmethionine synthase in Escherichia coli and optimization of enzyme-producing conditions[J]. Food and Fermentation Industries, 2020
, 46(10)
: 8
-13
.
DOI: 10.13995/j.cnki.11-1802/ts.023219
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