The method for simultaneous determination of 14 kinds of pesticide residues in Kaite mango was developed by QuEChERS with ultra-performance liquid chromatography-triple quadrupole mass spectrometry (UPLC-MS/MS). Ten grams samples were pretreated with an improved QuEChERS method, and then the target analytes were separated on a ZORBAX SB-C18 column with gradient elution using a mobile phase made up of 4mmol/L formic acid solution and methanol. Positive ion of electrospray ionization (ESI) and multiplereaction monitoring (MRM) mode were adopted in MS analysis. The concentration of 14 pesticides showed good linearity in the range of 4.0～200.0 μg/L (r＞0.999). The detection limits between 0.15 and 1.0 μg/kg, respectively. The spiked recovery rates were in the range of 86.5%～103.1%. The relative standard deviation (RSD) was 4.1%～11.5%. This method could meet the requirements for the determination of 14 kinds of pesticide residues in kaite mango.
TIAN Jinfeng
,
SHANG Yuanhong
. Determination of fourteen kinds of pesticide residues in Kaite mangoby QuEChERS with UPLC-MS/MS[J]. Food and Fermentation Industries, 2020
, 46(9)
: 272
-277
.
DOI: 10.13995/j.cnki.11-1802/ts.023204
[1] NTSOANE L M,ZUDE-SASSE, MAHAJAN P, et al. Quality assesment and postharvest technology of mango: A review of its current status and future perspectives[J]. Scientia Horticulturae, 2019, 249:77-85.
[2] 李昌宝,李丽,任二芳,等.果胶酶和纤维素酶对芒果出汁率及品质的影响[J].食品工业科技,2015, 36(13):217-219;224.
[3] GOWDA I N D, HUDDAR A G. Investigations on processing quality of some mango varieties, hybrids and their blends[J]. Journal of Food Science and Technology,2004,41(27):154-159.
[4] 周晓霜,胡雨林,苏畅,等.四川省攀枝花市芒果产业现状及优势分析[J].山西农经,2015(2):24-25.
[5] 张莉芝,青致刚,王建芳.浅谈保护和提升攀枝花市芒果质量对策[J].四川农业科技,2018(11):74-75.
[6] 张艳玲,徐志.我国与主要贸易国及国际组织芒果农药残留标准的分析研究[J].中国热带农业,2013(1):4-7.
[7] 乐渊,刘春华,黎舒怀,等.QuEChERS净化-超高效液相色谱-串联质谱法检测芒果中3种杀菌剂残留[J].农药学学报,2015,17(6):723-728.
[8] GB 2763—2019食品安全国家标准 食品中农药最大残留限量[S].北京:中国标准出版社,2019.
[9] 张嘉楠,曹秀梅,王爱卿.QuEChERS-超高效液相色谱-串联质谱法测定果蔬中57种农药残留量[J].化学分析计量,2018,27(4):60-65.
[10] 李萍萍,程景,黎舒怀,等.气相色谱/串联质谱法测定芒果中的腈苯唑和溴螨酯残留[J].热带作物学报,2015, 36(4):798-803.
[11] 汤永娇,袁宏球,朱珠,等.超高效液相色谱法测定芒果中啶酰菌胺和吡唑醚菌酯[J].食品科学,2014, 35(20):175-179.
[12] 张新忠,罗逢健,张芬,等.分散固相萃取净化气相色谱串联质谱法测定茶叶、西葫芦和芒果中噻螨酮和噻嗪酮残留量[J].分析化学,2012,40(11):1 686-1 692.
[13] ANASTASSIADES M, LEHHOTAY S J, STAJNBAHER D, et al. Fast and easy multiresidue method employing acetonitrile extraction/partitioning and “dispersive solid-phase extraction” for the determination of pesticide residues in produce[J]. Journal of Aoac International, 2003, 86(2): 412-431.
[14] 秦富,邓全道,李湧,等.QuEChERS-高效液相色谱-串联质谱法快速测定罗汉果中55种杀菌剂[J].色谱,2017,35(11):1 129-1 136.
[15] 叶倩,黄健祥,骆冲,等.QuEChERS-超高效液相色谱-串联质谱法快速测定3种叶菜中矮壮素和丙环唑残留[J].分析科学学报,2017,33(3):342-346.
[16] 李丹,王鹤佳,张玉洁,等.QuEChERS-高效液相色谱-荧光法检测鸡蛋中环丙沙星、达氟沙星、恩诺沙星和沙拉沙星的残留量[J].食品安全质量检测学报,2018,9(18):4 916-4 920.
[17] 高青珍. QuEChERS-色谱法快速测定蔬菜水果中的农药多残留[D].北京:中国农业科学院, 2014.
[18] 王晨晨.液相色谱——串联质谱对果蔬中农药残留测定的研究[D].济南:山东大学,2017.
[19] 李本昌.农药残留量实用检测方法手册[M].北京:化学工业出版社, 2001.
[20] GB 23200.8—2016 食品安全国家标准 水果和蔬菜中500种农药及相关化学品残留量的测定 气相色谱-质谱法[S].北京:中国标准出版社,2016.
[21] GB 23200.34—2016 食品安全国家标准 食品中涕灭砜威、吡唑醚菌酯、嘧菌酯等65种农药残留量的测定 液相色谱-质谱/质谱法[S].北京:中国标准出版社,2016.
[22] GB 23200.49—2016 食品安全国家标准 食品中苯醚甲环唑残留量的测定 气相色谱-质谱法[S].北京:中国标准出版社,2016.
[23] NY/T 1453—2007 蔬菜及水果中多菌灵等16种农药残留测定 液相色谱-质谱-质谱联用法[S].北京:中国标准出版社,2007.
[24] SN/T 0519—2010 进出口食品中丙环唑残留量的检测方法[S].北京:中国标准出版社,2010.
[25] GB 23200.46—2016 食品安全国家标准 食品中嘧霉胺、嘧菌胺、腈菌唑、嘧菌酯残留量的测定 气相色谱-质谱法[S].北京:中国标准出版社,2016.
[26] NY/T 788—2018 农药残留试验准则[S].北京:中国标准出版社,2018.
