To investigate the effects of ultrasonic mutagenesis on the content and activity of crude polysaccharides in mycelium and sporophore of Hericium erinaceus. The strains of Hericium erinaceus were subjected to ultrasonic mutagenesis at different times. After each mutagenized strain was initial screened by plate culture and then rescreened by liquid fermentation culture, a mutant strain of Hericium erinaceus with the highest biomass and the highest crude polysaccharide content was obtained finally. The original strain and the H-2 strain were then subjected to bag cultivation to obtain sporophores, and the crude polysaccharide content of the sporophores were compared. Finally, the antibacterial activities of the crude polysaccharides in the original strain and H-2 strain sporophores were compared. The crude polysaccharide contents of mycelium and sporophores of mutant strain H-2 were increased by 1.2% and 2.3%, respectively, and the crude polysaccharide of H-2 sporophore had significant antibacterial effect against Staphylococcus aureus. The content and antibacterial activity of crude polysaccharides in mycelium and sporophore can be improved by ultrasonic mutagenesis of Hericium erinaceus strains at an appropriate time.
ZHANG Shuai
,
CHENG Hao
,
QIU Caixia
,
CHEN Xianru
. Effect of ultrasonic mutation on crude polysaccharide of Hericium erinaceus[J]. Food and Fermentation Industries, 2020
, 46(2)
: 126
-130
.
DOI: 10.13995/j.cnki.11-1802/ts.020842
[1] 王晓玉, 蒋秋燕, 凌沛学, 等. 猴头菌活性成分及药理作用研究进展[J]. 中国生化药物杂志, 2010(1): 70-72.
[2] JIANG S J, WANG S H, SUN Y J, et al. Medicinal properties of Hericium erinaceus and its potential to formulate novel mushroom-based pharmaceuticals[J]. Applied Microbiology and Biotechnology, 2014, 98 (18): 7 661-7 670.
[3] FRIEDMAN M. Chemistry, nutrition, and health-promoting properties of Hericium erinaceus(Lion's Mane) mushroom fruiting bodies and mycelia and their bioactive compounds[J]. Journal of Agricultural and Food Chemistry, 2015, 63(32): 7 108-7 123.
[4] 张静, 张家臣, 高智席, 等. 猴头菇活性成分研究进展[J]. 南方农业, 2016(12): 186-188.
[5] WANG M X, GAO Y, XU D D, et al. Hericium erinaceus (Yamabushitake): A unique resource for developing functional foods and medicines[J]. Food & Function, 2014, 5(12):3 055-3 064.
[6] 张立秋. 激光诱变选育猴头多糖高产菌株的研究[J]. 人参研究, 2010,22(3): 16-17.
[7] 王楠, 任大明, 龚涛, 等.60Co-γ射线辐照诱变尖端菌丝选育猴头菌多糖高产菌株[J]. 中国食用菌, 2005,24(6): 37-39.
[8] 汪洁, 郑惠华, 刘广建, 等. 离子束注入诱变选育猴头菇高产菌株的研究[J]. 安徽农业科学, 2013,40(36):13 843-13 846.
[9] 高梦祥, 夏帆, 朱朋涛. 交变磁场对猴头菌生长及胞外多糖的影响[J]. 农业机械学报, 2009(2): 139-141;90.
[10] 于兰芳. 紫外线诱变猴头菌菌丝及变异菌株的筛选[D]. 保定: 河北农业大学, 2008.
[11] 雒祜芳, 曹辉. 超声诱变育种机理研究初探[C]. 中国声学学会会议论文集. 北京: 中国科学院声学所, 2009: 193-194.
[12] 王薇薇, 孟廷廷, 郭丹钊, 等. 食品加工中超声波生物学效应的研究进展[J]. 食品工业科技, 2015, 36(2): 379-383.
[13] 张帅, 程昊, 卢晓莹. 超声波辅助水提-蛋白酶法高效提取末水坛紫菜多糖[J]. 中国食品添加剂, 2019,30(1): 86-91.
[14] 韩丽丽, 刘敏. 诱变方法在微生物育种中的应用[J]. 酿酒, 2008, 35(3): 16-18.
[15] MASUKO T, MINAMI A, IWASAKI N, et al. Carbohydrate analysis by a phenol-sulfuric acid method in microplate format[J]. Analytical Biochemistry, 2005, 339(1):69-72.
[16] 秦培鹏. 猴头菌多糖提取工艺的研究[D]. 乌鲁木齐: 新疆农业大学, 2013.
[17] CHEN J J, ZHANG T, JIANG B, et al. Characterization and antioxidant activity of Ginkgo biloba exocarp polysaccharides[J]. Carbohydrate Polymers, 2012, 87(1): 40-45.
[18] ZHA S H, ZHAO Q S, CHEN J J, et al. Extraction, purification and antioxidant activities of the polysaccharides from maca (Lepidium meyenii)[J]. Carbohydrate Polymers, 2014, 111: 584-587.
[19] 宋海艳. 猴头菇栽培技术要点[J]. 中国农业信息, 2014, (12): 36.
[20] 陈庆榆, 缪成贵, 何华奇. 人工栽培猴头菌多糖提取工艺及抑菌作用研究[J]. 生物学杂志, 2012, 29(4): 89-91.
[21] 吴志明, 李公斌, 辛秀兰. 猴头菇多糖的提取工艺[J]. 食品研究与开发, 2011, 32(7):36-38.
