The endophytes separated and purified from mulberry twigs, using morphological observation, physiological and biochemical characteristics and molecular biology method to identify the species.And we have examined the activities of inhibition α-glucosidase andantioxidants of the different extractive phases of fermentation broth ofthe endophytes. The preliminary activities screening shows that three strains of endophyte Pseudomonas fermentation broth hasagood inhibiting activityα-glucosidase and antioxidant ,so we carried out the further study.Two kinds of solvents with different polarity were used, respectively, EAE, NBAE and WAE. And the fermentation liquid was extracted, and the three segment of activity was detected. The EAE(Ethyl Acetate Extract) of strain 4cfermentation broth has a good α-glucosidase activity and ABTS^+· free radical scavenging capacity. IC₅₀ respectively:1.749 mg/mL, 1.376 mg/mL.The EAE of strain 2s fermentation broth the strongest on the DPPH free radical scavenging, the IC₅₀ is 0.048 mg/mL.And the total reducing power was the bestest, and the ascorbic acid equivalent (AEE) is 191.627 mg AAE/g dw. This research is so vital to offer a reference for the use of mulberry endophytic bacteria producefunction food.