The
endophytes separated and purified from mulberry twigs, using morphological
observation, physiological and biochemical characteristics and molecular
biology method to identify the species.And we have examined the activities of
inhibition α-glucosidase andantioxidants of the different extractive phases of fermentation
broth ofthe endophytes. The preliminary activities screening shows that three strains of
endophyte Pseudomonas fermentation broth hasagood inhibiting activityα-glucosidase and antioxidant ,so we carried out the
further study.Two
kinds of solvents with different polarity were used, respectively, EAE, NBAE
and WAE. And the fermentation liquid was extracted, and the three segment of
activity was detected. The EAE(Ethyl Acetate Extract) of strain 4cfermentation
broth has a good α-glucosidase activity and ABTS+· free radical
scavenging capacity. IC50 respectively:1.749 mg/mL, 1.376 mg/mL.The
EAE of strain 2s fermentation broth the strongest on the DPPH free radical
scavenging, the IC50 is 0.048 mg/mL.And the total reducing power was
the bestest, and the ascorbic acid equivalent (AEE) is 191.627 mg AAE/g dw. This
research is so vital to offer a reference for the use of mulberry endophytic
bacteria producefunction food.
JIA Ya-nan et al
. The Inhibitionofα-glucosidaseand
Antioxidant Activityof Endophyte Metaboliteisolated from Mulberry[J]. Food and Fermentation Industries, 2017
, 43(11)
: 132
.
DOI: 10.13995/j.cnki.11-1802/ts.014921