The production of high-fructose syrup generates great quantities of waste liquor, which contains miscellaneous sugar. Efficient utilization of this mixed carbohydrate is an urgent problem that needs to be solved. In this study, we first determined the components of the mixed carbohydrate, which were glucose, fructose, and the linear glucans with a degree of polymerization ranging from 2-16, using HPLC, MALDI-MS, and FTIR. Specifically, it contained glucose 480 g/L, fructose 92 g/L, maltose 103.6 g/L and maltotriose 36.8 g/L. And the total sugar was 802.3 g/L. Compared with the standard carbon sources (glycerol and glucose), this mixed carbohydrate was used in the production of endo-β-1,3-glucanase by two common types of Pichia pastoris (Pichia pastoris PAOX1 and PGAP). The results showed that, for Pichia pastoris PAOX1, when the mixed carbohydrate was used as carbon source, cell density was lower than the standard carbon sources. And the maximum biomass (DCW) was 82.0 and 59.1 g/L, respectively. Meanwhile, the enzyme activity was also lower than control samples which reached 199.2 and 157.29 U/mL respectively. However, for Pichia pastoris PGAP, the fermentation effect of the mixed carbohydrate and glucose was roughly the same, which indicated that the mixed carbohydrate can be used a s a high-quality alternative carbon source for Pichia pastoris PGAP to produce endo-β-1,3-glucanase.
LIU Liping
,
WANG Li
,
ZHAN Xiaobei
,
ZHU Li
,
ZHENG Zhiyong
,
GAO Minjie
. Analysis of carbohydrate composition from high-fructose syrup waste and its utilization as a carbon source for Pichia pastoris fermentation[J]. Food and Fermentation Industries, 2020
, 46(3)
: 8
-13
.
DOI: 10.13995/j.cnki.11-1802/ts.022700
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