Enhanced butyrate production using waste Pichia pastoris as the efficient nitrogen source

  • CHEN Cheng ,
  • CHENG Wenjun ,
  • GONG Lipeng ,
  • DING Jian ,
  • SHI Zhongping
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  • 1 (School of Biotechnology, Jiangnan University, Wuxi 214122, China)
    2 (The Key Laboratory of Industrial Biotechnology, Ministry of Education (Jiangnan University), Wuxi 214122, China)

Received date: 2019-10-09

  Online published: 2020-03-13

Abstract

In this study, aiming at effectively utilizing solid waste Pichia pastoris and efficiently producing butyric acid, a novel butyric acid fermentation strategy was developed with 80 g/L corn-starch as the base medium and the pretreated waste yeast suspension as the efficient organic nitrogen source together with consecutive concentrated glucose solution feeding. In a 7 L anaerobic fermentor, the waste yeast suspension (250-400 g/L specification) was added into fermentation broth with a volume ratio of 1∶4 around 20 h to replace the expensive complex fermentation medium, followed by consecutively feeding concentrated glucose solution. With this strategy, butyric acid concentration, yield and butyric acid ratio over total organic acids (B/TA) reached high levels of 45 g/L, 0.40 and 0.90, respectively. This operation strategy could effectively utilize amino acids (4 g/L) originated from the waste yeast suspension to increase Clostridium tyrobutyricum growth rate and concentration, enhance butyric acid synthesis dependent NADH regeneration rate under high SO2-4 concentration environment created by waste yeast suspension addition. As a result, butyric acid fermentation performance was indirectly improved, at the same time bio-waste utilization/recirculation was realized.

Cite this article

CHEN Cheng , CHENG Wenjun , GONG Lipeng , DING Jian , SHI Zhongping . Enhanced butyrate production using waste Pichia pastoris as the efficient nitrogen source[J]. Food and Fermentation Industries, 2020 , 46(3) : 22 -29 . DOI: 10.13995/j.cnki.11-1802/ts.022726

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