Based on centrifugal force driving principle and recombinase-aid amplification technology, a centrifugal microfluidic chip system for rapid detection of cattle, sheep, pig, duck and chicken-derived ingredients was developed. The fan-shaped microfluidic chip, the sealing cover and the chip tray were designed and manufactured. A portable microfluidic rapid detection instrument integrating constant temperature control platform, fluid control module and photoelectric detection module was built, which using constant temperature amplification of nucleic acid and fluorescence detection methodology. This instrument can detect 5 samples at the same time, and 5 indicators can be detected simultaneously for each sample. The results showed that the minimum mass fraction detected in cattle, sheep, pig, duck and chicken-derived ingredients by centrifugal microfluidic chip system is 1%, 1%, 0.1%, 0.1% and 1% respectively. The centrifugal microfluidic chip system can realize semi-quantitative detection within 30 min. This system is fast, automatic and high-throughput which showed great potential for rapid detection of adulteration in meat products.
ZHOU Xinli
,
SHEN Bingyang
,
GAO Lijuan
,
KONG Bing
,
YE Jiaming
. Development of centrifugal microfluidic chip system for rapid detection of five animal-derived components[J]. Food and Fermentation Industries, 2020
, 46(3)
: 229
-234
.
DOI: 10.13995/j.cnki.11-1802/ts.021457
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