To further understand the metabolism of intracellular small-molecule compounds in Saccharomyces cerevisiae at different fermentation stages, targeted and non-targeted metabolomic approaches were utilized to analyze the metabolites of recombinant yeast strain N6076 and original strain Kh08 in the present study. The results of UPLC-Q-TOF/MS detection indicated that there were 11 and 10 species of intracellular small-molecule metabolites for strain N6076 and Kh08 at different fermentation time points, respectively, and most of which are lipids with high abundance. Among them, mevalonate-5-phosphate and cystine were the significantly different metabolites of the two strains. The synthesis time of small molecular compounds with molecular weight greater than 900 in metabolites of recombinant strain N6076 was 48h which later than that of the original strain Kh08. The result detected by LC-MS/MS method showed that the pentenyl mevalonate-5-phosphate (MVAP) concentration was 1.32 μg/mL when fermented with strain N6076 for 48 h, while no MVAP was detected at the same fermentation time point of strain Kh08. This study provides a basis for further investigation on the metabolism in yeast.
OU Ke
,
CHEN Fuxin
,
WANG Ting
,
FENG Guangwen
,
YAN Beibei
,
BAI Qiaoxiu
,
QIAN Weidong
,
MAO Peihong
. The analysis of intracellular differential metabolites of two Saccharomyces cerevisiaestrains based on metabonomics approach[J]. Food and Fermentation Industries, 2020
, 46(4)
: 39
-44
.
DOI: 10.13995/j.cnki.11-1802/ts.022214
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