It has been known that the furfural and acetic acid in the liquid corn stalk hydrolysate could reduce yeast activity. The purpose of this study was to screen a furfural and acetic acid-resistant, high -yield ethanol Saccharomyces cerevisiae through mutation and domestication. A mutant library derived from Saccharomyces cerevisiae xp was acquired through atmospheric and room-temperature plasma (ARTP) mutagenesis. Subsequently, the mutants were grown in corn stalk hydrolysate liquid medium. After 25 generations of continuous domestication, a strain, named xp2, was obtained. This domesticated mutant had a maximum biomass DCW of 3.71 g/L, which was 19.68% higher than the wild type (3.10 g/L). It also had a short growth cycle, with 36 hours, which was 20 hours shorter than the wild type. The concentrations of the furfural and acetic acid in supernatant were 1.43 g/L and 1.21 g/L, which were 62.17% and 26.67% lower than those of the wild type (3.78 g/L and 1.65 g/L) respectively. These results indicated that the transformation capacity of the furfural and acetic acid had been significantly improved. The ethanol yield and average yield of xp2 were 38.7 g/L and 0.806 g/(L·h), which increased by 17.12% and 27.71% than that of the wild type. In conclusion, the screened strain in this study had high transformation capacity for furfural and acetic acid, and the screening method provided valuable reference for screening high performance Saccharomyces cerevisiae.
CHEN Shengjie, GAO Xiang, YUAN Rongyu. Screening of high-yield ethanol Saccharomyces cerevisiae by the combination of atmospheric and room temperature plasma with corn stalk hydrolysate[J]. Food and Fermentation Industries, 2020, 46(4): 167-171. DOI: 10.13995/j.cnki.11-1802/ts.022416
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