To improve the utilization and detoxification capacity of Rhodopseudomonas capsulata on corn straw degradation products and convert the straw into feed, ethyl methane sulfonate, and ultraviolet rays were used as mutagens to mutagenize Rhodopseudomonas capsulata. The utilization capacity of the mutagenic strains on xylose and phenol was determined, and the pretreated corn straw was fermented to produce feed. The results showed that after the mutant strain (named FJM) was continuously subcultured for 10 times, the utilization rate of phenol and xylose were 97.73% and 94.44%, respectively, after 72 h of inoculation. When the mutant strain was used to ferment and transform the pretreated corn straw under the conditions of straw layer thickness of 4.5 cm, inoculum size of 3×108 pieces /mL, material-water ratio of 1:10 (straw g/ distilled water g), shaking table speed of 120 r/min, fermentation temperature of 31℃, and fermentation time of 6 d, the true protein content of fermented straw increased from 4.12% to 23.87%, crude cellulose decreased from 37.53% to 14.37%, fat increased from 4.43% to 6.51%, crude ash content decreased from 4.0% to 3.51%, and the moisture content rate of fermented dried grain was 9.97%. Therefore, FJM can increase the feed value of straw and has potential in the research of converting straw into high-protein feed.
LI Guoqiang
,
LUO Ping
,
KANG Jiawei
,
HU Ruizhou
,
ZHANG Jian
. Utilization of xylose and phenol and fermentation of straw to produce feed by mutagenic strains of Rhodopseudomonas capsulata[J]. Food and Fermentation Industries, 2020
, 46(6)
: 199
-204
.
DOI: 10.13995/j.cnki.11-1802/ts.022047
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