The lactase with the high galactosyl transferase activity is desired for the galacto-oligosaccharides (GOS) preparation. Bifidobacterium longum B1172 growing well on GOS but not on lactose-glucose-galactose was selected and its GOS-biomass correlation was established. A high-throughput screening method for transgalactosylation activity was subsequently developed and successfully applied for screening transgalactosylase-producing strains from the culture collection. Five strains producing lactase with the remarkably transgalactosyl activity were selected, which lay a foundation for further cloning and molecular evolution of the enzyme.
ZHAO Jihua
,
NIU Dandan
,
NOKUTHULA Peace Mchunu
,
TIAN Kangming
,
MIAO Jia
,
WANG Zhengxiang
. Establishment and preliminary application of a rapid screening method for lactase with high galactosyl transferase activity[J]. Food and Fermentation Industries, 2020
, 46(7)
: 17
-20
.
DOI: 10.13995/j.cnki.11-1802/ts.023697
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