The detection of common foodborne pathogens is an important prerequisite to ensure the safety of animal food. Consequently, developing effective methods for detecting common foodborne pathogens is necessary. In this study, surface-enhanced Raman scattering (SERS) method was applied to detect three common foodborne pathogens (Salmonella spp., Escherichia coli and Staphylococcus aureus) and compared with multiplex polymerase chain reaction (PCR) method. Specific primers were designed for multiplex PCR based on gene hilA, uidA and clfA, and followed by the amplification condition optimized and the specificity and sensitivity of the multiplex PCR system evaluated. Besides, the detection limits of the two methods were compared. The results showed that both the multiplex PCR and SERS methods could specifically detect the three foodborne pathogens. For the multiplex PCR, the minimum detection limit for microbes was 104 CFU/mL while for DNA was 50 pg per reaction. For SERS method, the detection limits for Salmonella sp., E. coli and S. aureus were 103 CFU/mL, 104 CFU/mL and 104 CFU/mL, respectively. In addition, the cumulative contribution rate of the first two principal components in the SERS method reached 93.8% according to the principal component analysis (PCA). In summary, the SERS method applied in this study revealed higher sensitivity for Salmonella sp., and shorter detection time than multiplex PCR. Therefore, the SERS method exhibited a good prospect in detecting foodborne pathogens.
ZHUANG Beibei
,
QI Zhao
,
ZHOU Zihui
,
HUANG Hao
,
SONG Xiangjun
,
SHAO Ying
,
TU Jian
. Establishment and comparison of foodborne pathogen detection models based on multiplex PCR and SERS[J]. Food and Fermentation Industries, 2020
, 46(7)
: 207
-212
.
DOI: 10.13995/j.cnki.11-1802/ts.023071
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