The content changes of soluble sugars (sucrose, maltose, glucose and fructose) and organic acids (lactic acid, acetic acid) as well as alcohols (glycerin, ethanol) during the fermentation of strong gluten wheat Zhengmai366, weak gluten wheat Zhengmai103, and medium gluten wheat Aikang58 were measured and analyzed using high performance liquid chromatography (HPLC). The results showed that sucrose was rapidly decomposed at the beginning of dough fermentation, and no sucrose was detected during the whole fermentation process. The content of maltose, glucose and fructose gradually decreased with the fermentation time, and the content would be below 0.5 mg/g dough after 3 h of fermentation; the content of glycerol and ethanol increased and was up to 8.383 and 3.179 mg/g after fermentation. The content of lactic and acetic acid increased gradually in the process of short-term fermentation, and fluctuated with the further increase of fermentation time. The changes of various substances in different gluten wheat flour doughs were similar but the change rates were different. There were differences in the changes of various metabolites content with fermentation time under different fermentation methods. The content of soluble sugars was the most significant change in the direct fermentation dough, while the change was small in the Laomiantuan fermentation dough, and the content of soluble sugar was the lowest in the sponge fermentation dough. The changes of acids, fermentable sugars and metabolites during fermentation can provide a basis for studying the effects of these substances on dough, the degree of fermentation, and the quality and flavor analysis of the final fermentation product.
YUE Qinghua
,
LIU Chong
,
LI Limin
,
ZHENG Xueling
. Effect of fermentation time and method on yeast metabolites in dough[J]. Food and Fermentation Industries, 2020
, 46(8)
: 27
-33
.
DOI: 10.13995/j.cnki.11-1802/ts.023252
[1] KIM Y, HUANG W, ZHU H, et al. Spontaneous sourdough processing of Chinese Northern-style steamed breads and their volatile compounds[J]. Food Chemistry, 2009,114(2):685-692.
[2] 杨浣漪. 传统酸面团中酿酒酵母和旧金山乳杆菌的种内多样性及其互作研究[D]. 杭州:浙江大学,2018.
[3] CHO I H, PETERSON D G. Chemistry of bread aroma: A review[J]. Food Science & Biotechnology, 2010, 19(3):575-582.
[4] MIRSAEEDGHAZI H, EMAM-DJOMEH Z, MOUSAVI S M A. Rheometric measurement of dough rheological characteristics and factors affecting it[J]. International Journal of Agriculture and Biology, 2008, 10(1): 112-119.
[5] NEWBERRY M P, PHAN-THIEN N, LARROQUE O R, et al. Dynamic and elongation rheology of yeasted bread doughs[J]. Cereal Chemistry, 2002, 79(6):874-879.
[6] GALAL A M, VARRIANO-MARSTON E, JOHNSON J A. Rheological dough properties as affected by organic acids and salt[J]. Cereal Chemistry, 1978, 55(5):683-691.
[7] POTUS J, POIFFAIT A, DRAPRON R. Influence of dough-making conditions on the concentration of individual sugars and their utilization during fermentation[J]. Cereal Chemistry, 1994, 71(5):505-508.
[8] SAHLSTRÖM S, PARK W, SHELTON D R. Factors influencing yeast fermentation and the effect of lmw sugars and yeast fermentation on hearth bread quality[J]. Cereal Chemistry, 2004, 81(3):328-335.
[9] MACARTHUR L A, D'APPOLONIA B L. Comparison of oat and wheat carbohydrates. I. Sugars[J]. Cereal Chemistry, 1979, 56(5):455-457.
[10] HENRY R J, SAINI H S. Characterization of cereal sugars and oligosaccharides[J]. Cereal Chemistry, 1989, 66(5):362-365.
[11] VERSTREPEN K J, ISERENTANT D, MALCORPS P, et al. Glucose and sucrose: Hazardous fast-food for industrial yeast[J]. Trends in Biotechnology, 2004, 22(10):531-537.
[12] JAYARAM V B, CUYVERS S, LAGRAIN B, et al. Mapping of Saccharomyces cerevisiae metabolites in fermenting wheat straight-dough reveals succinic acid as pH-determining factor[J]. Food Chemistry, 2013, 136(2):301-308.
[13] VILJOEN B C, LUES J F R. The microbial populations associated with post-fermented dough and compressed bakers yeast[J]. Food Microbiology, 1993, 10(5):379-386.
[14] 苏东民,胡丽花,苏东海,等.馒头发酵过程中酵母菌和乳酸菌的代谢作用[J].食品科学,2010,31(13):200-204.
[15] LOVEDAY S M, WINGER R J. Mathematical model of sugar uptake in fermenting yeasted dough[J]. Journal of Agricultural and Food Chemistry, 2007, 55(15):6 325-6 329.
[16] PARAMITHIOTIS S, CHOULIARAS Y, TSAKALIDOU E, et al. Application of selected starter cultures for the production of wheat sourdough bread using a traditional three-stage procedure[J]. Process Biochemistry, 2005, 40(8):2 813-2 819.
[17] 王大一.酵子制作老面馒头特性研究[D]. 郑州:河南工业大学,2016.
[18] 拱姗姗,刘长虹,张煌,等.发酵面糊对馒头面团流变学特性影响研究[J].食品工业科技,2015,36(9):53-56.
[19] 丁长河,戚光册,侯丽芬,等.传统老酵头馒头的品质特性[J].中国粮油学报,2007,22(3):17-20.
[20] 苏东海,胡丽花,苏东民,等.酵母菌和乳酸菌发酵老面团过程中对可溶性糖的代谢[J]. 食品工业科技,2011,32(3): 211-214.
[21] GABRIELA C G, DANIELA V. The influence of different forms of backery yeast Saccharomyces cerevisie type strain on the concentration of individual sugars and their utilization during fermentation[J]. Romanian Biotechnological Letters, 2010, 15(4):5 417-5 422.
[22] DECOCK P,CAPPELLE S. Bread technology and sourdough technology[J] Trends in Food Science & Technology,2005,16(1-3): 113-120.
[23] 杨浣漪. 传统酸面团中酿酒酵母和旧金山乳杆菌的种内多样性及其互作研究[D].杭州:浙江大学,2018.
[24] HEITMANN M, ZANNINI E, ARENDT E. Impact of Saccharomyces cerevisiae metabolites produced during fermentation on bread quality parameters: A review[J]. Critical Reviews in Food Science and Nutrition, 2018, 58(7): 1 152-1 164.