Beer foam stability, a key factor in assessing overall beer quality, is affected by proteinase A (PrA) in the fermentation broth. To weaken PrA excretion from Saccharomyces cerevisiae, the vacuolar sorting of PrA was strengthened by overexpression of Ca2+ transporter Gdt1. S. cerevisiae W303-1A was used as the parental strain, and Gdt1-overexpression strain was constructed by exploiting the in vivo homologous recombination-dependent DNA assembler. Fermentation experiments for the Gdt1-overexpression strain and the parental strain were performed under nitrogen-deficient condition, and PrA activity, and fermentation performance of the two strains were examined. Results showed that the intracellular PrA activities of the Gdt1-overexpression strain were increased by 12.59% and the extracellular PrA activities were decreased by 18.81% at the end of fermentation compared with those of the parental strain. Meanwhile, no obvious differences were found on the fermentation performance of the two strains. The research uncovers an effective method to decrease the extracellular secretion of S. cerevisiae PrA, which provides a theoretical reference for the screening of low-PrA-activity strains.
SONG Lulu
,
LIU Xiaohang
,
GUO Xuewu
,
CHEN Yefu
,
XIAO Dongguang
. Effect of Gdt1 overexpression on secretion of Saccharomyces cerevisiaeproteinase A[J]. Food and Fermentation Industries, 2020
, 46(9)
: 10
-17
.
DOI: 10.13995/j.cnki.11-1802/ts.023142
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