To identify the adulteration of sugar and water in apple juice, the δD, δ18O and δ13C values of water in fresh apple juice (FAJ) and adulterated apple juice(AAJ)were determined by isotope ratio mass spectrometry (IRMS). The results showed that δD and δ18O values of water in FAJ were significantly higher than those of exogenous water in AAJ and the δD, δ18O value was linear with the exogenous water content. Moreover, the δD, δ18O value declined with exogenous water content increased. The δ13C value of sugar component in FAJ showed that the fructose, glucose, and disaccharide content was -25.64‰--26.83‰, -25.01‰--26.36‰, and -22.41‰--23.24‰, respectively. Additionally, the percentage ranges of fructose, glucose and disaccharide in total sugar were 48.84%-52.39%, 14.34%-28.85%, and 10.47%-18.78%, respectively for FAJ, while the oligosaccharides were not detected. But the δ13C value and percentage in total sugar of disaccharide for AAJ were not in the above range. This study implies that the isotope ratios of δD, δ18O, δ13C are different between FAJ and AAJ, and can be used to distinguish the adulteration of fresh apple juice. It also provides an experimental basis for the application of isotope ratio mass spectrometry in the authentication detection of fresh apple juice.
LEI Jialei
,
TIAN Dan
,
XUE Jia
,
DENG Hong
,
MENG Yonghong
,
GUO Yurong
. Application of isotope ratio mass spectrometers of δD, δ18O, ＆ δ13Cin the authentication of fresh apple juice[J]. Food and Fermentation Industries, 2020
, 46(9)
: 234
-242
.
DOI: 10.13995/j.cnki.11-1802/ts.022905
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