A marine protease-producing fungal strain was screened from the local sea sediments in Xiamen. The morphology of this fungal strain was observed by scanning electron microscopy, and it was identified as Parengyodontium album HX2019006 by extracting the ITS sequence and comparing it in Genbank with the subsequent generation of the phylogenetic tree. This strain was capable of producing protease in seawater-based culture. We performed a single-factor optimization of the culture medium components and fermentation conditions to achieve an increase in protease yield. Three levels of maltose concentration, peptone concentration, and culture time were selected for the response surface methodology (RSM) optimization. Under the optimized culture medium and fermentation conditions, the protease activity in the fermentation broth was 71.1 U/mL, which was 125.7% higher than that under the initial conditions.
FU Qi
,
LIN Junjie
,
ZHUANG Zhixia
,
HUANG Huabin
,
SUN Enxian
,
GAN Meiyu
,
XIAO Yujuan
. Identification of the marine protease-producing fungus Parengyodontium album HX2019006 and the optimization of its fermentation conditions[J]. Food and Fermentation Industries, 2020
, 46(10)
: 185
-190
.
DOI: 10.13995/j.cnki.11-1802/ts.023581
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