In recent years, the increasing incidence of thrombotic diseases has seriously threatened human life and health. Some functional compounds in foods can increase the activity of human fibrinolytic system, thus gradually restore human's normal physiological state and reduce the tendency of thrombophilia. A novel fibrinolytic enzyme was purified from the fruiting body of Agrocybe aegerita by ammonium sulfate precipitation, Sephadex G-25 gel filtration, CM-Sepharose FF ion exchange, Source 15PHE hydrophobic chromatography and Mono S ion exchange. The fibrinolytic enzyme had a specific activity of 1 731.05 U/mg, with the purification fold of 246.94. The enzyme was named ACase and comprised two subunits. The molecular weights of the two subunits were estimated to be approximately 31.4 kDa and 21.2 kDa, respectively, by SDS-PAGE. Only one band appeared on Native-PAGE and it was found to show fibrinolytic activity. The analysis by Edman degradation method showed that the N-terminal sequences of the two subunits were AIVTQTNAPWGL and SNADGNGHGTHV, respectively. The enzyme was identified as a newfibrinolyticenzymebysequencecomparisoninGenBank. ACase could degrade the three chains (α, β and γ) of fibrinogen in order and exhibited the functionality of anticoagulant in vitro. Agrocybe aegerita has the potential application as functional food ingredient in food industry.
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