Based on the optimized pretreatment method and instrument detection conditions, an inhibitory ion chromatography method was established to simultaneously detect the contents of nitrate and nitrite in livestock, poultry and their products. The samples were homogenized, extracted by ultrasound, and then separated by static separation. After removing protein, fat, and other substances by dichloromethane extraction, the supernatant was filtered and purified. And then the purified samples were analyzed by ion chromatography (IC) using KOH solution as eluent and detected by a suppressed conductivity detector. The results showed that the separation degree of nitrate and nitrite was good, the quantitation limit were 0.3 and 0.2 mg/kg and the detection limit were 0.09 and 0.06 mg/kg. Nitrate and nitrite showed good linear relationship in the range of 0.2-2.0 mg/kg and 0.02-0.2 mg/kg, respectively. Three concentration levels, low, medium and high, were labeled and measured six times in parallel. The average nominal recovery rates of nitrate and nitrite in pig, cattle, sheep, chicken and duck were between 77.0%-106.1%, 77.3%-101.0%, 65%-97.5%, 80.0%-103.3% and 90.5%-99.2%, respectively. And the average relative standard deviation (RSD) was less than 8.75%. The method established in this study is simple and fast, with accurate and reliable and can be used for simultaneous detection of nitrate and nitrite in livestock, poultry and their products.
SUN Yanqin
,
ZHANG Fangfang
,
SHEN Juan
,
WU Xinyi
,
LI Chunbao
,
CHEN Ying
,
WANG Wei
. Conductance detection of nitrate and nitrite in livestock and poultrymuscle by inhibitory ion chromatography[J]. Food and Fermentation Industries, 2020
, 46(11)
: 264
-268
.
DOI: 10.13995/j.cnki.11-1802/ts.023361
[1] 姬建生, 高敬铭, 符锋, 等. 抑制电导-离子色谱法同时测定肉制品中亚硝酸盐和硝酸盐[J]. 中国食物与营养, 2010(10): 50-52.
[2] 张宝勇. 六种腌制菜中亚硝酸盐含量及食用安全性评价研究[J]. 中国调味品, 2012, 37(9): 96-98.
[3] 顾翔宇, 郭军. 硝酸盐/亚硝酸盐检测方法的研究进展[J]. 中国食物与营养, 2016, 22(3): 24-28.
[4] 张蕾, 张天慧, 张志华, 等. 离子色谱法检测熟肉制品中亚硝酸盐含量的影响因素分析[J]. 食品安全导刊, 2016, 9(64): 121-123.
[5] 杨翰南, 纪宏, 张宏宏, 等. 离子色谱法对发酵肉制品中亚硝酸盐含量的检测[J]. 品牌与标准化, 2017(11): 76-77.
[6] 姚敬, 杭义萍, 钟志雄, 等. 在线渗析-离子色谱联用同时测定熟肉制品中的亚硝酸盐和硝酸盐[J]. 食品科学, 2010, 31(2): 187-190.
[7] GB 2760—2014食品添加剂使用标准[S]. 北京: 中国标准出版社, 2014.
[8] 沈银梅, 白婕. 离子色谱法测定卤肉制品中亚硝酸盐和硝酸盐[J]. 肉类工业, 2016(12): 41-43.
[9] 方素珍, 陈春祝, 苏晓鹏. 离子色谱法测定肉制品中的硝酸盐及亚硝酸盐[J]. 中国卫生检验杂志, 2011, 21(3): 762;771.
[10] 程慰先. 离子色谱法改进后检测肉制品中亚硝酸盐含量[J]. 河南预防医学杂志, 2016, 27(8): 592-593;636.
[11] 林静佳, 胡国媛, 李荔, 等. 紫外分光光度法快速测定乳制品中硝酸盐含量[J]. 中国卫生检验杂志, 2012,22(8): 1 757-1 759;1 763.
[12] SOBHANARDAKANI S, FARMANY A, ABBASI S, et al. A new catalytic-spectrophotometric method for quantification of trace amounts of nitrite in fruit juice samples [J]. Environmental Monitoring and Assessment, 2013, 185(3): 2 595-2 601.
[13] 王燕, 刘素美, 任景玲, 等. 化学发光法测定天然水体中低浓度硝酸盐和亚硝酸盐的含量[J]. 海洋科学, 2011, 35(5): 95-99.
[14] 张会亮, 苗贝贝, 黄传峰, 等. 液相色谱-串联质谱法测定火腿肠和咸菜中亚硝酸盐含量[J]. 食品安全质量检测学报, 2018, 9(20): 5 313-5 318.
[15] 战旭梅, 刘靖, 刘萍. 高效液相色谱法测定香肠中亚硝酸盐含量[J]. 食品与机械, 2014, 30(6): 72-74.
[16] 包海英, 李晓明, 王飞, 等. 液相色谱法检测食品中溴酸盐、亚硝酸盐和硝酸盐[J]. 食品研究与开发, 2013, 34(22): 72-74.
[17] 陈华. 顶空-气相色谱法测定食品中亚硝酸盐[J]. 世界最新医学信息文摘, 2019, 19(51): 319-320.
[18] 何浩, 陈幸莺, 孙映球, 等. 顶空-气相色谱法测定食品中亚硝酸盐[J]. 食品与机械, 2017, 33(1): 55-58.
[19] 程辉. 气相色谱法测定食品中亚硝酸盐[J]. 河南预防医学杂志, 2010, 21(4): 262-263.
[20] GB 5009.33-2016食品安全国家标准食品中亚硝酸盐与硝酸盐的测定[S]. 北京: 中国标准出版社, 2016.
[21] 张翠芬. 食品添加剂硝酸盐、亚硝酸盐检测方法研究进展[J]. 中国食品添加剂, 2014(6): 152-155.
[22] 姚秀娟. 英蓝超滤-离子色谱法同时测定熟肉制品中硝酸盐和亚硝酸盐含量[J]. 河南预防医学杂志, 2015, 26(5): 360-361;370.
[23] 刘岚松, 康绍英, 张丽, 等. 离子色谱法测定肉制品中亚硝酸盐与硝酸盐的改进研究[J]. 食品与机械, 2015, 31(2): 83-86.
[24] 付饶, 卫碧文, 张烨雯, 等. 高效液相色谱-串联质谱法快速测定塑料食品接触材料中5种脂肪胺[J]. 分析试验室, 2019, 38(9): 1 095-1 099.
[25] 王硕, 刘广福, 孙蕊, 等. 离子色谱法测定肉制品中的硝酸盐和亚硝酸盐[J]. 食品科技, 2014, 39(2): 302-305.
[26] 王瑞, 徐军, 张瑞良, 等. 基于分子印迹技术检测鸡肉中16种喹诺酮药物残留[J]. 分析科学学报, 2019, 35(2): 152-158.
[27] 陆炀. 靖江猪肉脯生产工艺的研究[J]. 中国调味品, 2018, 43(4): 148-149;153.
