Dansyl chloride is used as a derivatization reagent for the quantitative determination of putrescine produced by microbial fermentation. However, the determination of putrescine was significantly interfered by the complex culture broth, leading to significant deviations in the results. The influence of fermentation broth on the detection of putrescine was studied to achieve more accurate determination of putrescine. A quantitative method using the calibration curves was successfully established with the detection error less than 7.96%, based on the precolumn derivatization of dansyl chloride. The noteworthy improvement was that it could significantly eliminate the interfering effects from culture broth without removing impurities, thus offering a feasible solution for quantitative determination of biosynthesized putrescine, and laying a solid foundation for strains selection and further industrial biosynthesis of putrescine.
HUANG Dixuan
,
LI Guohui
,
MAO Yin
,
ZHAO Yunying
,
ZHOU Shenghu
,
DENG Yu
. A quantitative method for microbial-based putrescine determination[J]. Food and Fermentation Industries, 2020
, 46(12)
: 231
-236
.
DOI: 10.13995/j.cnki.11-1802/ts.023394
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