The contents of Mn, Zn, Sr and Cd in algae were determined by ultrasonic enzyme extraction and inductively coupled plasma mass spectrometry(ICP-MS). The optimum extraction method was determined by comparing the extraction efficiency of pepsin, cellulase and dilute HCl (pH 1.0) for Mn, Zn, Sr and Cd. The influence of pH value, ultrasonic temperature, enzyme quality, ultrasonic time and liquid to solid ratio on the extraction efficiency of Mn, Zn, Sr and Cd in ultrasonic pepsin extraction was investigated by single factor test, and the optimal extraction conditions were obtained. The results showed that the extraction efficiency of elements by ultrasonic pepsin extraction was the best, and the extraction efficiency was above 93%. The optimum technological conditions for single factor extraction of Mn, Zn, Sr and Cd from ultrasonic pepsin extraction were as follows: pH 1.0, pepsin 20 mg, liquid to solid ratio at 7∶0.2(mL∶g), and ultrasonic treating 20 min at 45 ℃. The linear relationships of Mn, Zn, Sr and Cd were good, and the correlation coefficients were all higher than 0.999 6. The detection limit was in the range of 0.001 5-0.086 2 mg/kg. The relative standard deviation was lower than 3.51%, and the recovery rate was 89.5%-107.8%. The results were verified by national standard material (GBW10025), which was accurate and reliable. In conclusion, this method is simple, rapid, green, environmentally friendly, and is suitable for the determination of Mn, Zn, Sr and Cd in algae samples.
FAN Xiaoxu
,
ZHOU Meili
,
YANG Chunhua
,
YANG Wei
. Determination of Mn, Zn, Sr and Cd in algae by ICP-MS with ultrasonic enzyme extraction[J]. Food and Fermentation Industries, 2020
, 46(12)
: 271
-276
.
DOI: 10.13995/j.cnki.11-1802/ts.023756
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