A method for the determination of α-solanine and α-chaconine in potato and its products by high performance liquid chromatography with diode array detector was established. After being extracted by acidified methanol, the sample was centrifuged with anhydrous sodium sulfate and anhydrous magnesium acetate to absorb the excess water. The sample was separated by Hubble C18 liquid chromatography column (250 mm× 4.6 mm, 5 μm). Acetonitrile and 0.02 mol/L potassium dihydrogen phosphate solution were used as the mobile phases. The flow rate was 0.2 mL/min, the column temperature was 30 ℃, the sample volume was 10 μL, and the detection range of the detector was 190-810 nm, the detection wavelength was 195 nm. The linear correlation coefficient r was 0.999 3-0.999 8 in the concentration range of 0.2-200 μg/mL, the detection limit of the method was 0.5 mg/kg, the quantitative limit was 1.7 mg/kg, the recoveries of 4, 6 and 10 μg/mL were 98%-107%, and the relative standard deviation was 0.05%-0.44%. The method has the advantages of simple pretreatment, high sensitivity, good reproducibility and recovery, good precision and accuracy, and could be used for the analysis and detection of α-solanine and α-chaconine in potato and its products.
REN Xingquan
,
LIU Pan
,
SU Ju
,
WANG Rong
,
ZENG Wenjin
,
ZHOU Li
,
SHI Rong
,
LI Tingting
,
DUAN Ting
. Determination of α-solanine and α-chaconine in potatoes and its products by high performance liquid chromatography-diode array detector[J]. Food and Fermentation Industries, 2020
, 46(12)
: 277
-282
.
DOI: 10.13995/j.cnki.11-1802/ts.023826
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