In order to compare the difference between the automatic colony counting (Microbio) method and the ordinary plate counting method, ten bacteria strains were added to food samples at high, medium and low concentration. And the standard methods of GB 4789.2—2016 and GB 4789.15—2016 were used for colony counting as control. Data accuracy, quantitative limit, the detection time, personnel operation error and different media were compared and analyzed. In terms of data accuracy, personnel operation error and different media, the recovery rate of the two methods were all over 70% and the relative standard deviation (RSD) ≤0.35. When the detection limit ≥5 CFU/mL, the recovery rate ≥70% and the RSD ≤0.35 respectively. The results showed that no significant difference was found between the automatic colony count method (Microbio) and the ordinary plate count method and the quantitative limit could reach 5 CFU/mL. But in terms of the detection time, the automatic colony count method (Microbio) was superior to the ordinary plate method.
GUO Jia
,
WANG Ping
,
ZHOU Jifu
,
ZHAO Xiaomei
,
LIU Jifeng
,
CHEN Ying
. A comparative study between Microbio method and plate counting methodon colony counting[J]. Food and Fermentation Industries, 2020
, 46(13)
: 237
-243
.
DOI: 10.13995/j.cnki.11-1802/ts.023190
[1] GB 4789.2—2016. 食品安全国家标准 食品微生物学检验 菌落总数测定[S].北京:中国标准出版社,2017.
[2] 刘德,吴鑫,杨洁,等. 婴幼儿米粉中菌落总数检测方法的比较[J].食品安全质量检测学报, 2018, 9(23): 6 090-6 094.
[3] 谭静,平洋,朱海华. 金黄色葡萄球菌两种检测方法的比较研究[J].食品安全质量检测学报, 2016, 7(6): 2 277-2 280.
[4] 孙霞,蒋廉华,卢新. 3种方法检测食品中菌落总数的比较[J].中国卫生检验杂志, 2014, 24(9): 44-45; 48.
[5] 赵立冬,赵红阳,石业娇,等. 3MTM PetrifilmTM 快速菌落总数测试片法与食品中菌落总数检测国标方法(GB 4789.2—2010)的比较[J].中国微生态学杂志, 2018, 30(10): 1 203-1 210; 1 216.
[6] 谢雪钦. 两种方法测定菌落总数和大肠菌群的比较研究[J].食品工业, 2015, 36(10): 283-285.
[7] 谢翌冬. 生物阻抗法检测猪肉新鲜度及其模型预测研究[D].南京:南京农业大学, 2016.
[8] 杜寒春,叶开富,刘绍刚,等. 电阻抗法快速测定巴氏杀菌牛乳中菌落总数[J].中国乳品工业, 2017, 45(4): 56-58; 61.
[9] 侯玉柱,田雨,柯润辉,等.ATP生物发光法快速测定物体表面的菌落总数[J].食品与发酵工业,2015,41(2):217-220.
[10] BARI M L, KAWASAKI S. Encyclopedia of Food Microbiology[M]. Elsevier Inc, 2014: 269-279.
[11] RAYMOND Y, CHAMPAGNE C P. The use of flow cytometry to accurately ascertain total and viable counts of Lactobacillus rhamnosus in chocolate[J]. Food Microbiology, 2015, 46: 176-183.
[12] GB 4789.15—2016. 食品安全国家标准 食品微生物学检验 霉菌和酵母计数[S].北京:中国标准出版社,2017.
[13] SCHILLING M W. Chemical Analysis | Sampling and Statistical Requirements[M]. Encyclopedia of Meat Sciences, 2014, 1: 187-192.
[14] GB/T 32465—2015. 化学分析方法验证确认和内部质量控制要求[S].北京:中国标准出版社,2016.
[15] ROBSON C A, ADRIANO A S, VIVIANE P M, et al. Co-expression, purification and characterization of the lipase and foldase of Burkholderia contaminans LTEB11[J].International Journal of Biological Macromolecules, 2018, 116: 1 222-1 231.
[16] TAGELE S B, KIM S W, LEE H G, et al. Effectiveness of multitrait Burkholderia contaminans KNU17BI1 in growth promotion and management of banded leaf and sheath blight in maize seedling[J]. Microbiological Research, 2018, 214: 8-18.
[17] 梅仕良. 豆制品中菌落总数的流式细胞术检测研究[D].上海:上海师范大学, 2019.
[18] 张兰,徐红,翁文川,等. 流式细胞技术检测酸性饮料中菌落总数的研究[J].食品工业科技, 2018, 39(4): 235-239.
[19] 张浩. ATP生物荧光法快速检测化妆品中菌落总数的研究[J].工业微生物, 2017, 47(5):53-56.
[20] 于瑞莉,茆伟伟,龚丽芳.5种平板计数培养基质量分析[J].中国卫生检验杂志,2016,26(22):3 240-3 244.
[21] TSUTA M, SASAKI Y, TAKEUCHI I, et al. Use of multivariate analysis for the improvement in prediction accuracy of bacterial aerobic plate count by flow cytometry[J]. Elsevier Ltd, 2014, 55(2): 472-476.
[22] LUCAS C D, FEDERICO F,RIBEIRO LUIZ E B, et al. Label-free counting of Escherichia coli cells in nanoliter droplets using 3D printed microfluidic devices with integrated contactless conductivity detection[J].Analytica Chimica Acta., 2019, 1 071: 36-43.
[23] JIANG Xingxing,LIU Shuping,YANG Minghui, et al. Amperometric Genosensor for Culture Independent Bacterial Count[J]. Sensors and Actuators B: Chemical, 2019, 299: 126 944.
