To increase the production of ε-poly-L-lysine (ε-PL) of Streptomyces albulus and reduce its production cost further, overexpression of ε-PL synthetase gene pls in S.albulus combined with the addition of the precursor and energy co-factor during fermentation was employed in this study. The results showed that the pls gene transcription in S. albulus M-Z18/pIB139-pls was up-regulated by 16.8-fold, and the ε-PL production in shake flask and 5 L fermenter reached (2.74±0.23) g/L and 40.62 g/L (144 h), respectively. In order to further meet the needs of the engineered strain for precursor and energy substances during the biosynthesis of ε-PL, the concentrations of L-lysine and ATP were optimized in shake-flask fermentation, and finally the fed-batch fermentation mode with the addition of 5.0 g/L L-lysine and 1.0 mmol/L adenosine triphosphate (ATP) in a 5 L fermenter was developed. The production of ε-PL reached 45.09 g/L (144 h) and increased by 20.8% than that of the control strain S. albulus M-Z18/pIB139. As a result, it is an effective strategy to improve ε-PL production of S. albulus by overexpression of pls gene and the addition of L-lysine and ATP during fermentation. The result provides a reference for improving ε-PL production.
WANG Ze
,
WANG Kaifang
,
HU Yangfan
,
MAO Zhonggui
,
CHEN Xusheng
. Enhanced ε-poly-L-lysine production through overexpression of pls gene combined with precursor feeding in Stretomyces albulus[J]. Food and Fermentation Industries, 2020
, 46(14)
: 1
-6
.
DOI: 10.13995/j.cnki.11-1802/ts.023475
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