After preliminarily isolated from Anhui traditional fermented soybean food--fairy soybean, by blood-agar method, five strains were further examined by oil spreading, inhibitory test and detection of genes related to lipopeptide synthesis, and one strain BS6-2 producing Surfactin was screened out. Strain BS6-2 was identified as Bacillus subtilis by 16S rDNA gene sequencing analysis. The metabolite of BS6-2 was preliminarily identified as lipopeptide by Fourier transform infrared spectrometry (FT-IR). Using HPLC and ultra-performance liquid chromatography-electron spray ionization-quadrupole-time-of-flight/mass spectrometry (UPLC-ESI/Q-TOF MS) and compared with standards, the lipopeptide was further determined as three Surfactin homologues. The possible sequence was β-OH-C13-15-Glu-Leu-Leu-Val-Asp-Leu-Leu(Ile). The Surfactin lipopeptides produced by strain BS6-2 showed stable emulsification capability and also exhibited antifungal activity against Aspergillus niger. These results suggest that B. subtilis BS6-2 has potential application prospect.
CHEN Zhina
,
XUE Yongzhen
,
YE Tao
,
LIU Tian
,
SHEN Yeqiao
. Screening of lipopeptide-producing strains from fairy soybean and the characteristic of its lipopeptide products[J]. Food and Fermentation Industries, 2020
, 46(14)
: 48
-53
.
DOI: 10.13995/j.cnki.11-1802/ts.023671
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