Research progress of ester-producing yeast and its key enzymes for esters synthesis

  • PENG Liying ,
  • LIU Gongliang ,
  • FEI Yongtao ,
  • YU Jieyu ,
  • LIU Rui ,
  • BAI Weidong ,
  • WANG Lichang ,
  • JIA Aijuan
Expand
  • 1(College of Light Industry and Food Science, Zhongkai University of Agriculture and Engineering, Guangzhou 510225, China)
    2(Guangzhou Eagle-coin Food Group Co., Ltd., Guangzhou 510655, China)
    3(Guangdong Delicious Fresh Seasoning Food Co. Ltd., Zhongshan 528437, China)

Received date: 2020-04-15

  Online published: 2020-08-17

Abstract

Ester-producing yeast is a kind of microorganism that can produce ester substances, and it plays an important role in food fermentation industry. In this paper, the breeding methods of ester-producing yeast were reviewed, the advantages and disadvantages of total ester determination method were compared, and the key enzymes and genes of yeast for esters synthesis were discussed. The screening and development trend of ester-producing yeast in food fermentation industry were also prospected. This paper provides theoretical basis for the application of ester-producing yeast in food fermentation industry.

Cite this article

PENG Liying , LIU Gongliang , FEI Yongtao , YU Jieyu , LIU Rui , BAI Weidong , WANG Lichang , JIA Aijuan . Research progress of ester-producing yeast and its key enzymes for esters synthesis[J]. Food and Fermentation Industries, 2020 , 46(14) : 275 -282 . DOI: 10.13995/j.cnki.11-1802/ts.024198

References

[1] 于洋,粟春燕,陈晶瑜.3株葡萄酒非酿酒酵母的生长与发酵特性[J].食品科学,2015,36(19):106-111.
[2] HOLT S, MUKHERJEE V,LIEVENS B,et al.Bioflavoring by non-conventional yeasts in sequential beer fermentations[J].Food Microbiology,2018,72:55-66.
[3] ZHANG Qi,HUO Nairui,WANG Yuanhui,et al.Aroma-enhancing role of Pichia manshurica isolated from Daqu in the brewing of Shanxi Aged Vinegar[J].Intertional Journal of Food Properties,2017,20(9):2 169-2 179.
[4] 陈嘉,冯志宏,施俊凤.传统山西老陈醋增香酵母菌的筛选及其特性研究[J].保鲜与加工,2020,20(1):120-126.
[5] 刘景,王欣,辛红鸿,等.非酿酒酵母的筛选及其发酵低醇苹果汁的研究[J].中国食品学报,2017,17(1):134-140.
[6] 冯杰.埃切假丝酵母产香机理及其对酱油风味的影响[D].无锡:江南大学,2012.
[7] 王晓丹,陈美竹,班世栋,等.茅台大曲中酵母的分离、鉴定及其功能初探[J].食品科学,2017,38(4):51-57.
[8] 陆振群.产酯类酵母菌的选育[D].芜湖:安徽工程大学,2012.
[9] 杨盛春,杨明惠,高云涛.自动电位滴定法测不同类酒中的总酸总酯[J].广东化工,2013,40(8):145;138.
[10] 易新华.白酒中总酯测定方法的探讨[J].酿酒科技,2008,(7):120-121.
[11] 刘千,陈黎,李华,等.采用气相色谱测定宜宾浓香型原酒中总酯方法研究标准[J].中国标准化,2016(20):36-37.
[12] TIAN Jiyuan.Determination of several flavours in beer with headspace sampling-gas chromatography[J].Food Chemistry,2010,123(4):1 318-1 321.
[13] YE Mengqi,YUE Tianli,YUAN Yahong,et al.Application of FT-NIR spectroscopy to apple wine for rapid simultaneous determination of soluble solids content, pH, total acidity, and total ester content[J].Food and Bioprocess Technology,2014,7(10):3 055-3 062.
[14] LÖBS A K,LIN J L,COOK M,et al.High throughput, colorimetric screening of microbial ester biosynthesis reveals high ethyl acetate production from Kluyveromyces marxianus on C5, C6, and C12 carbon sources[J].Biotechnology Journal,2016,11(10):1 274-1 281.
[15] 张春杰,宋春雪.微波皂化法在山西老陈醋总酯测定中的应用[J].中国调味品,2016,41(7):120-121.
[16] 卢亭,何计龙,夏慧丽,等.水浴静置皂化法测定白酒中的总酯[J].食品研究与开发,2016,37(23):154-156.
[17] 罗小叶,邱树毅,陆安谋,等.酱香大曲产香酵母的分离及鉴定[J].食品与发酵工业,2016,42(12):26-31.
[18] GARAVAGLIA J,HABEKOST A,BJERK T R,et al.A new method for rapid screening of ester-producing yeasts using in situ HS-SPME[J].Journal of Microbiological Methods,2014,103:1-2.
[19] 李锐利.高产乙酸乙酯酵母的选育及在清香型小曲酒中的应用研究[D].湖北:湖北工业大学,2011.
[20] 王春玲,宋茜,侯丽华,等.耐盐产酯酵母的选育及发酵性能研究[J].中国酿造,2010(6):20-24.
[21] YE Mengqi,YUE Tianli,YUAN Yahong,et al.Production of yeast hybrids for improvement of cider by protoplast electrofusion[J].Biochemical Engineering Journal,2013,81:162-169.
[22] 林小江.低甲醇高总酯酿酒酵母的选育及其应用研究[D].广州:华南理工大学,2014.
[23] 葛峻伶,郭莹,刘小二,等.一次敲除BAT2同时过表达Lg-ATF1对啤酒酵母产醇酯的影响[J].现代食品科技,2019,35(6):171-176;272.
[24] LÖBS A K,ENGEL R,SCHWARTZ C,et al.CRISPR-Cas9-enabled genetic disruptions for understanding ethanol and ethyl acetate biosynthesis in Kluyveromyces marxianus[J].Biotechnology for Biofuels,2017,10(1):164.
[25] LI Ping,GE Junling,GAO Yingying,et al.A seamless gene deletion method and its application for regulation of higher alcohols and ester in Baijiu Saccharomyces cerevisiae[J].BioMed Research International,2019:6 723 849.
[26] AKAMATSU S,KAMIYA H,YAMASHITA N,et al.Effects of aldehyde dehydrogenase and acetyl-CoA synthetase on acetate formation in sake mash[J].Journal of Bioscience and Bioengineering,2005,90(5):555-560.
[27] VADALI R V,BENNETT G N,SAN K Y.Applicability of CoA/acetyl-CoA manipulation system to enhance isoamyl acetate production in Escherichia coli[J].Metabolic engineering,2004,6(4):294-299.
[28] DONG Jian,WANG Pengfei,FU Xiaomeng,et al.Increase ethyl acetate production in Saccharomyces cerevisiae by genetic engineering of ethyl acetate metabolic pathway[J]. Journal of industrial microbiology & biotechnology,2019,46(6):801-808.
[29] 郑楠,郭庆焕,何亚辉,等.乙酰辅酶A含量对酿酒酵母乙酸乙酯合成的影响[J].中国酿造,2018,37(5):150-156.
[30] PLATA C,MAURICIO J C,MILLAÁN C,et al.Influence of glucose and oxygen on the production of ethyl acetate and isoamyl acetate by a Saccharomyces cerevisiae strain during alcoholic fermentation[J].World Journal of Microbiology and Biotechnology,2005,21(2): 115-121.
[31] LIAN Jiazhang,ZHAO Huimin.Recent advances in biosynthesis of fatty acids derived products in Saccharomyces cerevisiae via enhanced supply of precursor metabolites[J].Journal of Industrial Microbiology and Biotechnology,2015,42(3):437-451.
[32] CHEN Yefu,LUO Werwei,GONG Rui,et al.Improved ethyl caproate production of Chinese liquor yeast by overexpressing fatty acid synthesis genes with OPI1 deletion[J].Journal of Industrial Microbiology & Biotechnology,2016,43(9):1 261-1 270.
[33] ZHANG Jianwei,ZHANG Cuiying,WANG Jianxun,et al.Expression of the gene Lg-ATF1 encoding alcohol acetyltransferases from brewery lager yeast in Chinese rice wine yeast[J].Proceedings of the 2012 International Conference on Applied Biotechnology,2014,249:43-51.
[34] VERSTREPEN K J, VAN L S D,VANDERHAEGEN B,et al.Expression levels of the yeast alcohol acetyltransferase genes ATF1, Lg-ATF1, and ATF2 control the formation of a broad range of volatile esters[J]. Applied and Environmental Microbiology,2003,69(9):5 228-5 237.
[35] SHEN H Y,MOONJAI N,VERSTREPEN K J,et al.Immobilization of Saccharomyces cerevisiae induces changes in the gene expression levels of HSP12,SSA3,and ATF1 during beer fermentation[J].Journal of the American Society of Brewing Chemists, 2003,61(4):175-181.
[36] VERSTREPEN K J,DERDELINCKX G,DUFOUR J P,et al.The Saccharomyces cerevisiae alcohol acetyl transferase gene ATF1 is a target of the cAMP/PKA and FGM nutrient-signalling pathways[J].Fems Yeast Research,2003,4(3):285-296.
[37] LI Wei,CUI Danyao,WANG Jianhui,et al.Overexpression of different alcohol acetyltransferase genes with BAT2 deletion in Saccharomyces cerevisiae affects acetate esters and higher alcohols[J].European Food Research & Technology,2018,244(3):555-564.
[38] 李锋,陈叶福,郭建,等.过表达EHT1基因对酿酒酵母己酸乙酯生产能力的影响[J].现代食品科技,2014,30(3):93-98;176.
[39] KRUIS A J,LEVISSON M,MARS A E,et al.Ethyl acetate production by the elusive alcohol acetyltransferase from yeast[J]. Metabolic Engineering,2017,41:92-101.
[40] LOSER C,RRIT T,KEIL P,et al.Studies on the mechanism of synthesis of ethyl acetate in Kluyveromyces marxianus DSM 5422[J].Applied microbiology and biotechnology,2015,99(3):1 131-1 144.
[41] LÖBS A K,SCHWARTZ C, THORWALL S,et al.Highly multiplexed CRISPRi repression of respiratory functions enhances mitochondrial localized ethyl acetate biosynthesis in Kluyveromyces marxianus[J].ACS synthetic biology,2018,7(11):2 647-2 655.
[42] SAERENS S,VERSTREPEN K J,VAN L S D,et al.The Saccharomyces cerevisiae EHT1 and EEB1 genes encode novel enzymes with medium-chain fatty acid ethyl ester synthesis and hydrolysis capacity[J].The Journal of Biological Chemistry,2006,281(7):4 446-4 456.
[43] SAERENS S,VERBELEN P J,VANBENEDEN N,et al.Monitoring the influence of high-gravity brewing and fermentation temperature on flavour formation by analysis of gene expression levels in brewing yeast[J].Applied Microbiology and Biotechnology,2008,80(6):1 039-1 051.
[44] KUSANO M,SAKAI Y,KATO N,et al.Hemiacetal dehydrogenation activity of alcohol dehydrogenases in Saccharomyces cerevisiae[J].Bioscience, Biotechnology and Biochemistry,1998,62(10):1 956-1 961.
[45] 刘港,李洁,任津莹,等.产乳酸乙酯酿酒酵母菌株的构建[J].中国酿造,2018,37(7):72-77.
[46] YURIMOTO H,LEE B,YASUDA F,et al.Alcohol dehydrogenases that catalyse methyl formate synthesis participate in formaldehyde detoxification in the methylotrophic yeast Candida boidinii[J].Yeast,2004,21(4):341-350.
[47] VANRIJSWIJCK I M H,KRVIS A J,WOLKERSROOIJACKERS J C M,et al.Acetate-ester hydrolase activity for screening of the variation in acetate ester yield of Cyberlindnera fabianii, Pichia kudriavzevii and Saccharomyces cerevisiae[J].LWT,2019,104:8-15.
[48] ZHANG Jianwei,ZHANG Cuiying, DAI Longhai,et al.Effects of overexpression of the alcohol acetyltransferaseencoding gene ATF1 and disruption of the esterase-encoding gene IAH1 on the flavour profiles of Chinese yellow rice wine[J].International Journal of Food Science and Technology,2012,47(12):2 590-2 596.
[49] SCHNEIDERANGER H,KOOB J,POLTINGER S,et al.Gene expression in wheat beer yeast strains and the synthesis of acetate esters[J].Journal of the Institute of Brewing,2016,122(3):403-411.
[50] MA Jinming,LU Qianda,YUAN Ye,et al.Crystal structure of isoamyl acetate-hydrolyzing esterase from Saccharomyces cerevisiae reveals a novel active site architecture and the basis of substrate specificity[J].Proteins,2011,79(2):662-668.
Outlines

/