High-salinity adaptive domestication and characterization of high lutein producing microalgae Chlorella sorokiniana FZU60

  • MA Ruijuan ,
  • TANG Zhuzhen ,
  • ZHAO Xurui ,
  • XIE Youping ,
  • CHEN Jianfeng
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  • 1(Technical Innovation Service Platform for High Value and High Quality Utilization of Marine Organism,Fuzhou University,Fuzhou 350108,China)
    2(Fujian Engineering and Technology Research Center for Comprehensive Utilization of Marine Products Waste,Fuzhou University,Fuzhou 350108,China)
    3(Fuzhou Industrial Technology Innovation Center for High Value Utilization of Marine Products,Fuzhou University,Fuzhou 350108,China)
    4(College of Chemistry and Chemical Engineering,Xiamen University,Xiamen 361005,China)

Received date: 2020-04-08

  Revised date: 2020-05-07

  Online published: 2020-08-15

Abstract

Improving the salt-tolerance of microalgae can reduce the dependence on fresh water resources in the culture process and thus reduce the production cost of microalgae-based products. The high-salinity adaptive domestication of high lutein producing microalgae Chlorella sorokiniana FZU60 was carried out,and the changes in cell growth and cell composition during adaptive domestication were analyzed. The results showed that a salt-tolerant strain growing well under 50 g/L salinity was obtained after high salinity adaptive domestication. The cell size of salt-tolerant strain was bigger,and the color was darker than that of wild strain. In addition,the mass fraction of lipid and protein of salt-tolerant strain was increased,and the mass fraction of pigment was similar,while the mass fraction of carbohydrate was decreased,compared with that of wild strain. At 30 g/L salinity,the salt-tolerant strain could reach higher biomass productivity of 767.89 mg/(L·d) and lutein productivity of 5.62 mg/(L·d),which were 20.34% and 20.31% higher than that of wild strain,respectively. Hence,the salt-tolerant strain obtained greater lutein production ability,which can provide a suitable algal species source for microalgae-based lutein production and reduce the production cost by using seawater.

Cite this article

MA Ruijuan , TANG Zhuzhen , ZHAO Xurui , XIE Youping , CHEN Jianfeng . High-salinity adaptive domestication and characterization of high lutein producing microalgae Chlorella sorokiniana FZU60[J]. Food and Fermentation Industries, 2020 , 46(15) : 32 -38 . DOI: 10.13995/j.cnki.11-1802/ts.024169

References

[1] RUDALL P J.An introduction to plant structure and development: Plant anatomy for the twenty-first century[J]. Annals of Botany,2011,36(2): 520.
[2] SUN Z,LI T,ZHOU Z G,et al.Microalgae as a source of lutein: Chemistry,biosynthesis,and carotenogenesis[J]. Microalgae Biotechnology,2016,153: 37-58.
[3] LIN J H,LEE D J,CHANG J S.Lutein production from biomass: marigold flowers versus microalgae[J]. Bioresource Technology,2015,184: 421-428.
[4] ACIÉN F G,FERNNDEZ J M,MAGN J J,et al. Production cost of a real microalgae production plant and strategies to reduce it[J]. Biotechnology Advances,2012,30(6): 1 344-1 353.
[5] BOROWITZKA M A,MOHEIMANI N R.Sustainable biofuels from algae[J]. Mitigation and Adaptation Strategies for Global Change,2013,18(1): 13-25.
[6] DRAGOSITS M,MATTANOVICH D.Adaptive laboratory evolution-principles and applications for biotechnology[J]. Microbial Cell Factories,2013,12(1): 64.
[7] KATO Y,HO S H,VAVRICKA C J,et al.Evolutionary engineering of salt-resistant Chlamydomonas sp. strains reveals salinity stress-activated starch-to-lipid biosynthesis switching[J]. Bioresource Technology,2017,245: 1 484-1 490.
[8] FERNNDEZ-SEVILLA J M,FERNNDEZ F G A,GRIMA E M. Biotechnological production of lutein and its applications[J]. Applied Microbiology and Biotechnology,2010,86(1): 27-40.
[9] XIE Y P,LI J,MA R,et al.Bioprocess operation strategies with mixotrophy/photoinduction to enhance lutein production of microalga Chlorella sorokiniana FZU60[J]. Bioresource Technology,2019,290: 121 798.
[10] XIE Y P,HO S H,CHEN C N,et al.Phototrophic cultivation of a thermo-tolerant Desmodesmus sp. for lutein production: effects of nitrate concentration,light intensity and fed-batch operation[J]. Bioresource Technology,2013,144: 435-444.
[11] XIE Y P,ZHAO X R,CHEN J F,et al.Enhancing cell growth and lutein productivity of Desmodesmus sp. F51 by optimal utilization of inorganic carbon sources and ammonium salt[J]. Bioresource Technology,2017,244: 664-671.
[12] XIE Y P,LU K Y,ZHAO X R,et al. Manipulating nutritional conditions and salinity-gradient stress for enhanced lutein production in marine microalga Chlamydomonas sp.[J]. Biotechnology Journal,2019,14(4): 1 800 380.
[13] 谢友坪,赵旭蕊,阳需求,等. 脉冲式添加氮源对耐温微藻Desmodesmus sp. F51细胞生长和细胞组成的影响[J]. 食品科学,2017,38(14): 64-70.
[14] 李君兰,杨澜,吴潇霞,等. 葡萄糖酸钙采前处理对鲜枣果实低温贮藏品质及活性氧代谢的影响[J]. 食品与发酵工业,2019,45(7): 144-150.
[15] CHEN C Y,JESISCA,HSIEH C,et al.Production,extraction and stabilization of lutein from microalga Chlorella sorokiniana MB-1[J]. Bioresource Technology,2016,200: 500-505.
[16] TAYLOR K L,BRACKENRIDGE A E,VIVIER M A,et al.High-performance liquid chromatography profiling of the major carotenoids in Arabidopsis thaliana leaf tissue[J]. Journal of Chromatography A,2006,1121(1): 83-91.
[17] SUI Y X,MUYS M,VAN DE WAAL D B,et al. Enhancement of co-production of nutritional protein and carotenoids in Dunaliella salina using a two-phase cultivation assisted by nitrogen level and light intensity[J]. Bioresource Technology,2019,287: 121 398.
[18] WHO/FAO/UNU Expert Consultation. Protein and amino acid requirements in human nutrition introduction[C].World Health Organization Technical Report,2007.
[19] 王宝贝,李丽婷,刘磊,等. 烘焙处理对小球藻营养成分及其抗氧化活性的影响[J]. 食品与发酵工业,2019,45(4): 147-151.
[20] ALYABYEV A J,LOSEVA N L,GORDON L K,et al.The effect of changes in salinity on the energy yielding processes of Chlorella vulgaris and Dunaliella maritima cells[J]. Thermochimica Acta,2007,458(1-2): 65-70.
[21] EL-SHEEKH M,ABOMOHRA A E F,HANELT D. Optimization of biomass and fatty acid productivity of Scenedesmus obliquusas a promising microalga for biodiesel production[J]. World Journal of Microbiology and Biotechnology,2013,29(5): 915-922.
[22] ZHANG L,PEI H,CHEN S,et al.Salinity-induced cellular cross-talk in carbon partitioning reveals starch-to-lipid biosynthesis switching in low-starch freshwater algae[J]. Bioresource Technology,2018,250: 449-456.
[23] KHONA D K,SHIROLIKAR S M,GAWDE K K,et al.Characterization of salt stress-induced palmelloids in the green alga Chlamydomonas reinhardtii[J]. Algal Research,2016,16: 434-448.
[24] SHAH M M,LIANG Y,CHENG J J,et al.Astaxanthin-producing green microalga Haematococcus pluvialis: from single cell to high value commercial products[J]. Frontiers in Plant Science,2016,7: 531.
[25] SUBRAMANYAM R,JOLLEY C,THANGARAJ B,et al.Structural and functional changes of PSI-LHCI supercomplexes of Chlamydomonas reinhardtii cells grown under high salt conditions[J]. Planta,2010,231(4): 913-922.
[26] KENT M,WELLADSEN H M,MANGOTT A,et al.Nutritional evaluation of Australian microalgae as potential human health supplements[J]. PloS One,2015,10(2): e0118985.
[27] ZHILA N O,KALACHEVA G S,VOLOVA T G.Effect of salinity on the biochemical composition of the alga Botryococcus braunii Kütz IPPAS H-252[J]. Journal of Applied Phycology,2011,23(1): 47-52.
[28] XU X Q,BEARDALL J.Effect of salinity on fatty acid composition of a green microalga from an antarctic hypersaline lake[J]. Phytochemistry,1997,45(4): 655-658.
[29] VAZQUEZ-DUHALT R,ARREDONDO-VEGA B O. Haloadaptation of the green alga Botryococcus braunii[J]. Phytochemistry,1991,30(9): 2 919-2 925.
[30] ISHIKA T,BAHRI P A,LAIRD D W,et al.The effect of gradual increase in salinity on the biomass productivity and biochemical composition of several marine,halotolerant,and halophilic microalgae[J]. Journal of Applied Phycology,2018,30(3): 1 453-1 464.
[31] ISHIKA T,MOHEIMANI N R,BAHRI P A,et al.Halo-adapted microalgae for fucoxanthin production: Effect of incremental increase in salinity[J]. Algal Research,2017,28: 66-73.
[32] HO S H,CHAN M C,LIU C C,et al.Enhancing lutein productivity of an indigenous microalga Scenedesmus obliquus FSP-3 using light-related strategies[J]. Bioresource Technology,2014,152: 275-282.
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