Food and Fermentation Industries >

2020 , Vol. 46 >Issue 15: 32 - 38

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.024169

High-salinity adaptive domestication and characterization of high lutein producing microalgae Chlorella sorokiniana FZU60

  • MA Ruijuan ,
  • TANG Zhuzhen ,
  • ZHAO Xurui ,
  • XIE Youping ,
  • CHEN Jianfeng
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  • 1(Technical Innovation Service Platform for High Value and High Quality Utilization of Marine Organism,Fuzhou University,Fuzhou 350108,China)
    2(Fujian Engineering and Technology Research Center for Comprehensive Utilization of Marine Products Waste,Fuzhou University,Fuzhou 350108,China)
    3(Fuzhou Industrial Technology Innovation Center for High Value Utilization of Marine Products,Fuzhou University,Fuzhou 350108,China)
    4(College of Chemistry and Chemical Engineering,Xiamen University,Xiamen 361005,China)

Received date: 2020-04-08

  Revised date: 2020-05-07

  Online published: 2020-08-15

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Abstract

Improving the salt-tolerance of microalgae can reduce the dependence on fresh water resources in the culture process and thus reduce the production cost of microalgae-based products. The high-salinity adaptive domestication of high lutein producing microalgae Chlorella sorokiniana FZU60 was carried out,and the changes in cell growth and cell composition during adaptive domestication were analyzed. The results showed that a salt-tolerant strain growing well under 50 g/L salinity was obtained after high salinity adaptive domestication. The cell size of salt-tolerant strain was bigger,and the color was darker than that of wild strain. In addition,the mass fraction of lipid and protein of salt-tolerant strain was increased,and the mass fraction of pigment was similar,while the mass fraction of carbohydrate was decreased,compared with that of wild strain. At 30 g/L salinity,the salt-tolerant strain could reach higher biomass productivity of 767.89 mg/(L·d) and lutein productivity of 5.62 mg/(L·d),which were 20.34% and 20.31% higher than that of wild strain,respectively. Hence,the salt-tolerant strain obtained greater lutein production ability,which can provide a suitable algal species source for microalgae-based lutein production and reduce the production cost by using seawater.

Key words： Chlorella sorokiniana; lutein; high-salinity; adaptive domestication; cell composition

Cite this article

MA Ruijuan , TANG Zhuzhen , ZHAO Xurui , XIE Youping , CHEN Jianfeng . High-salinity adaptive domestication and characterization of high lutein producing microalgae Chlorella sorokiniana FZU60[J]. Food and Fermentation Industries, 2020 , 46(15) : 32 -38 . DOI: 10.13995/j.cnki.11-1802/ts.024169

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