D-arabitol, a functional pentitol, is widely used in food, pharmaceutical and chemical industries. To increase D-arabitol production, Candida parapsilosis SK26.002 were mutated using diethyl sulfate to obtain mutants with high D-arabitol production. After screening and genetic stability verification, mutant strain D137 was obtained and the yield of D-arabitol increased from 21.64 g/L to 27.29 g/L. The fermentation media was optimized as 300 g/L glucose, 5 g/L yeast extract, 25 g/L urea, 0.1 g/L FeCl3·6H2O, 0.02 g/L CaCl2 based on the single factor test and orthogonal experiments. Under optimal conditions, D-arabitol production was 87.58 g/L, and reached the highest production of 96.68 g/L at 120 h in a 3 L fermenter. The results showed that mutagenesis and fermentation optimization were effective approaches to increase D-arabitol production.
ZHENG Simeng
,
ZHANG Tao
,
JIANG Bo
. Mutation breeding of Candida parapsilosis producing D-arabitol and fermentation optimization[J]. Food and Fermentation Industries, 2020
, 46(16)
: 121
-126
.
DOI: 10.13995/j.cnki.11-1802/ts.024098
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