The detection method of carmine, sunset yellow, allure red, brilliant blue, acid red, basic orange 2, basic orange 21, basic orange 22, acid orange 2, auramine O in beverages, prepared wines, and dried soy products O using high performance liquid chromatography were established. Methanol: water (8∶2) were used as the extraction solvent to directly extract the pigment from the sample. The high-performance liquid chromatograph equipped with a diode array detector for the determination and the column was Agela Venusil XBP-C18 XBP-C18 (250 mm×4.6 mm, 5 μm). The following condition was used in this study including 449 nm, 485 nm, 254 nm, and 628 nm absorption wavelengths; column temperature at 25 °C with 1 mL/min flow rate. Mobile phase: 20 mmol/L ammonium acetate solution-methanol for elution. According to the methodological verification, all the chromatographic peak resolutions were>1.5. The correlation coefficients within the linear range of 0.5 mg/kg-125 mg/kg were all> 0.999; the recovery rate of the liquid samples was > 85% and the recovery rate of the solid samples was > 75%. Based on the calculation of the standard addition of beverages, the intra-day precision (RSD%) of 10 pigments was 0.76%-4.10% (n=6), and the intra-day precision (RSD%) was 1.09%-5.29% (n=6). The results showed that the method had good accuracy, sensitivity and precision, and could meet the testing requirements of the food testing industry.
GUO Yonghui
,
ZHAO Lianxing
,
KE Zehua
,
ZHANG Jiying
,
LIU Guiqiao
. Determination of colorants and industrial dyes in food by high performance liquid chromatography[J]. Food and Fermentation Industries, 2020
, 46(17)
: 259
-263
.
DOI: 10.13995/j.cnki.11-1802/ts.024562
[1] BONAN S, FEDRIZZI G, MENOTTA S, et al. Simultaneous determination of synthetic dyes in foodstuffs and beverages by high-performance liquid chromatography coupled with diode-array detector[J]. Dyes and Pigments,2013, 99(1): 36-40.
[2] DE ARAU′JO SIQUEIRA BENTO W, LIMA BP, PAIM APS. Simultaneous determination of synthetic colorants in yogurt by HPLC[J]. Food Chem,2015,183: 154-160.
[3] LI R,WANG Y,TAN J, et al. Simultaneous determination of synthetic edible pigments in beverages by titania-based RP-HPLC[J]. Arabian Journal of Chemistry, 2020, 13(2): 3 875-3 881.
[4] 王宏伟,刘素丽,赵梅,等.食品中非法添加工业染料危害的研究进展[J].食品安全质量检测学报,2019,10(01): 1-7.
[5] 李巧玲,田晶.食用合成色素的安全性评价及对策[J].食品工业,2017,38(11): 268-271
[6] GB2760—2014,食品安全标准 食品添加剂使用标准[S].北京:中国标准出版社,2014.
[7] 张兰天,王红,王利军等.高效薄层色谱法同时快速检测饮料中26种人工色素的研究[J].食品工业,2014,35(8):238-242.
[8] YI J, ZENG L, WU Q, et al. Sensitive simultaneous determination of synthetic food colorants in preserved fruit samples by capillary electrophoresis with contactless conductivity detection[J]. Food Analytical Methods. 2018,11(6): 1 608-1 618.
[9] 宋新,纪双利,杨丽,等.示波极谱法在食品合成食用色素测定中的应用[J].中国食品卫生杂志,2009,21(5):422-423.
[10] 杨园园,许乾丽,黎殊. HPLC法同时测定辣椒面中碱性橙2,21,22和酸性橙Ⅱ的含量[J].中国调味品,2018,43(6): 165-167.
[11] 刘莉萍,毕思远,龚达浩,等. HPLC法测定食品中的防腐剂和合成着色剂[J].食品工业,2018,39(1):266-269.
[12] 迟秋池.多种食品基质中12种水溶性人工合成色素测定方法研究[D].上海:上海交通大学,2014.
[13] 王蕾.蜜饯中添加剂的检测方法优化及应用[D].邯郸:河北工程大学,2018.
[14] 郝鹏飞,徐琴,吕宁,等.液相色谱同时测定腌渍蔬菜中的10种合成色素[J].食品研究与开发,2013,34(22):64-67.
[15] 刘敏,李小林,别玮,等.固相萃取-高效液相色谱法同时测定调味品中15种工业合成染料[J].色谱,2011,29(2): 162-167.
[16] 宋丹萍.食品中10种色素的高效液相色谱—质谱检测方法研究[D].成都:四川师范大学,2015.
[17] 张海君,何微娜,倪承珠,等.超高效液相色谱-串联质谱法测定虾干中12种工业染料[J].中国卫生检验杂志,2019,29(6):658-661;668.
[18] 刘思洁,范明,姜楠,等.超高效液相色谱-串联质谱法测定食品中酸性橙Ⅱ、碱性橙2和碱性嫩黄[J].中国卫生检验杂志,2013,23(12):2 593-2594;2638.
[19] 胡江涛,何成艳,刘兴睿,等.固相萃取-超高效液相色谱串联质谱法同时测定食品中8种禁用色素[J].安徽农业科学,2020,48(01): 204-207.
[20] GB 5009.35—2016 食品安全国家标准 食品中合成着色剂的测定[S].北京:中国标准出版社,2016.
[21] GB/T 23496—2009 食品中禁用物质的检测 碱性橙染料 高效液相色谱法[S].北京:中国标准出版社,2009.
[22] GB/T 22221—2008 食品中果糖、葡萄糖、蔗糖、麦芽糖、乳糖的测定 高效液相色谱法[S]. 北京:中国标准出版社,2008.
