Lycopene is a natural pigment with strong oxidation resistance. It has been widely used in food, health products and cosmetic industries. In this study, three copies of three different heterologous CrtE genes coding geranylgeranyl diphosphate synthase (GGPPS) were integrated into the genome DNA of Saccharomyces cerevisiae YPH499△gal80. Four CrtB genes coding phytoene synthase (CrtB) and four CrtI genes coding phytoene dehydrogenase (CrtI) from diverse species were then combined into 16 different lycopene-producing episomal plasmids and then transformed into the above-mentioned mutant strains. Finally, the lycopene detection method was also explored and optimized. The absorbance in five different solvents was analyzed. The optimal solvent, wavelength, detection limit and linear range were defined. One optimal combination mutant was screened (CrtE from Taxus x media, CrtB from Pantoea agglomerans and CrtI from Blakeslea trispora) through visual observation of the color of colonies and micro plate reader detection. According to the absorbance at 472 nm by micro plate reader, the highest titer of lycopene reached 1.33 mg/g DCW (dry cell weight). The screened recombinant strain in this work enhanced the productivity of lycopene-producing strains and provided the basis for construction of cell factories for lycopene production.
ZHOU Qinqin
,
XU Sha
,
ZHOU Jingwen
. Optimization of lycopene biosynthesis pathway genes and product determination[J]. Food and Fermentation Industries, 2020
, 46(18)
: 24
-32
.
DOI: 10.13995/j.cnki.11-1802/ts.023762
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