Screening and identification of γ-lactamase-producing strains

  • SUN Kang ,
  • LI Panpan ,
  • YU Shuang ,
  • CHI Naiyu
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  • 1(School of Life Science and Biotechnology, Dalian University, Dalian 116622, China)
    2 (Liaoning Technology of Marine Microbiological Engineering Research Center, Dalian 116622, China)

Received date: 2020-02-21

  Revised date: 2020-03-31

  Online published: 2020-10-23

Abstract

In order to achieve the separation of pharmaceutical intermediates (±)γ-lactam, two highly stereoselective γ-lactamase-producing strains were screened from the Bohai sea mud. They can be used to separate (±)γ-lactam by biological enzyme method, supplying the demand of pharmaceutical industry for single configuration γ-lactam. They were isolated by plate screening method combined with liquid chromatography method and identified by morphological observation combined with molecular biological technology. The pilot production study was also carried out to study their application capacity. The results showed that the screened strain LP7 could selectively degrade (+)γ-lactam and was identified as Arthrobacter agilis, the screened strain LY9 could selectively degrade (-)γ-lactam and was identified as Psychrobacter faecalis. Through pilot production study, these two strains prepared (-)γ-lactam and (+)γ-lactam, respectively, with high optical purity. Therefore, these two strains have the potential of industrial application.

Cite this article

SUN Kang , LI Panpan , YU Shuang , CHI Naiyu . Screening and identification of γ-lactamase-producing strains[J]. Food and Fermentation Industries, 2020 , 46(18) : 117 -121 . DOI: 10.13995/j.cnki.11-1802/ts.023712

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