The detection and control of beer spoilage and organic matter residues in beer brewing process is one of the main effective methods to prevent microbial contamination and ensure beer quality. This study aimed to establish a rapid assessment method of microbial contamination risk during beer brewing based on the adenosine triphosphate (ATP) bioluminescence method. A portable ATP fluorescence detector was used to detect the contamination of Lactobacillus brevis on the surface, in the gradient diluted L. brevis suspension and the residual organic matter in beer. The correlation analysis of the relative fluorescence unit (RLU) and the total number of L. brevis on the surface, bacterial concentration in water and beer organic matter residues was conducted, respectively. Three kinds of different prediction models were established. The correlation coefficients of these “matched” combinations including total surface bacteria, bacterial concentration in water and beer organic matter residual prediction model, were all greater than 0.9 (P<0.05). Among them, beer organic matter residual prediction model was highly relevant to RLU (R2=0.994). The prediction model of total L. brevis on the surface, concentration of L. brevis in water and residual organic matter in beer was established based on the portable ATP fast fluorescence detection system, which can provide effective real-time monitoring for the hygiene inspection in the beer brewing process, and also provides theoretical support for the application of luminescence method in food quality and safety.
GUO Liyun
,
XIANG Jie
,
XIE Xin
,
SHI Yamin
. Rapid detection of Lactobacillus brevis in beer with ATP bioluminescence[J]. Food and Fermentation Industries, 2020
, 46(18)
: 232
-235
.
DOI: 10.13995/j.cnki.11-1802/ts.024135
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