Food and Fermentation Industries >

2020 , Vol. 46 >Issue 19: 17 - 22

DOI: https://doi.org/10.13995/j.cnki.11-1802/ts.023524

Strengthening glyoxylate bypass and the TCA cycle improved 1,3-propanediol production in Klebsiella pneumoniae

  • XIE Mengmeng ,
  • HU Huitao ,
  • LU Xinyao ,
  • ZONG Hong ,
  • ZHUGE Bin
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  • 1(Key Laboratory of Carbohydrate Chemistry and Biotechnology,Ministry of Education,Jiangnan University,Wuxi 214122,China);
    2(The Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, School of Biotechnology,Wuxi 214122, China);
    3(Research Centre of Industrial Microbiology, Jiangnan University, Wuxi 214122, China)

Received date: 2020-02-05

  Revised date: 2020-02-29

  Online published: 2020-11-02

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Abstract

Acetate overflow and low tricarboxylic acid cycle (TCA cycle) activity inhibit the bioproduction of 1,3-propanediol from glycerol by Klebsiella pneumoniae. To relieve the overflow in acetyl-CoA node, iclR encoding isocitrate lyase regulator was deleted, sdhC encoding succinate dehydrogenase and mdh encoding malate dehydrogenase were overexpressed. Deletion of iclR decreased the accumulation of acetate by 41% and increased the 1,3-propanediol titer by 8%. Further overexpression of sdhC or mdh decreased the accumulation of 2,3-butanediol by 47% or 52% and improved the yield of 1,3-propanediol by 7% or 8%. Co-expression of sdhC and mdh improved the glycerol consumption and increased the 1,3-propanediol titer by 11%. The biomass of K. pneumoniae Lric-sdhC-mdh was significantly improved and the 1,3-propanediol titer reached 77.2 g/L with a molar yield of 0.69 mol/moL in fed-batch fermentation. These results indicated that activating glyoxylate bypass by disruption of iclR and reinforcement of the TCA cycle by overexpression of sdhC and mdh decrease acetate accumulation and increase glycerol consumption, which are favorable for the synthesis of 1,3-propanediol.

Key words： iclR; sdhC; mdh; glyoxylate bypass; TCA cycle; Klebsiella pneumoniae; 1,3-propanediol

Cite this article

XIE Mengmeng , HU Huitao , LU Xinyao , ZONG Hong , ZHUGE Bin . Strengthening glyoxylate bypass and the TCA cycle improved 1,3-propanediol production in Klebsiella pneumoniae[J]. Food and Fermentation Industries, 2020 , 46(19) : 17 -22 . DOI: 10.13995/j.cnki.11-1802/ts.023524

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