Bioinformatics methods were used to reveal the classification and phylogenetic relationship of xylose isomerase gene family in the Leguminosae. Thirty-four xylose isomerase gene sequences in the Leguminosae were obtained from NCBI website. Their subcellular localizations, motifs, conserved regions, primary structures, secondary structures, and phylogenetic relationships were analyzed with WoLF PSORT, MEME, NCBI, ExPASy, PredictProtein and MEGA, respectively. The results showed that the length of the 34 sequences ranged from 1 410 bp to 1 440 bp, and the main subcellular localizations were mitochondria, cytoplasm and endoplasmic reticulum. Furthermore, motif analysis demonstrated that the 34 sequences could be divided into 15 different motifs. Primary structure analysis demonstrated that the ranges of molecular mass and isoelectric points (pH) were around 52 952.24-54 793.33 Da and 5.52-7.95, respectively. The random coil had the highest proportion through analyzing the secondary structure data. Phylogenetic analysis demonstrated that the xylose isomerase genes could be divided into five groups. This study provides a theory basis for the further research of the xylose isomerase gene family in the Leguminosae.
YANG Ping
,
ZHANG Bianjiang
,
WANG Like
,
FU Qingquan
,
TANG Ning
,
CHEN Quanzhan
. Analysis of xylose isomerase gene family in Leguminosae[J]. Food and Fermentation Industries, 2020
, 46(19)
: 23
-27
.
DOI: 10.13995/j.cnki.11-1802/ts.024709
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