The anaerobic co-digestion technology was used to deal with the food waste and excess sludge. The continuous operation of CSTR reactor was carried out by gradually increasing the organic loading rate, and lasted for 128 days. The results showed that the biogas yield, methane yield and system stability were various under different organic loading rates. The value of pH, VFA and the content of ammonia nitrogen decreased with the increase of reaction loading rate, while the activities of proteases, amylases and dehydrogenases were fluctuated during the reaction process. When the organic loading rate of the reactor was 2.5, 5.0, 7.5 and 10 g VS/(L·d), the biogas yield was 71 670, 311 180, 361 840 and 362 610 mL, respectively. The results indicated that the optimal operation loading rate of CSTR was 7.5 g VS/(L·d), and the reaction system will collapse when the loading rate was too high.
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