Distiller's grains, the largest by-product of Baijiu (Chinese liquor) production, is rich in proteins, and has potential to be protein resource for industrial application. The added value can be enhanced, if the distiller's grains is properly used. Drying is an important way to avoid the distiller's grains mildew during the time of high temperature and rain. Taking the strong flavor distiller's grains as research object, the protein fractionation was analyzed, the ethanol-alkaline method and acetic-acid method were used to extract the prolamin of distiller's grains before and after drying. Analysis and comparison of the yield and purity, molecular weight, amino acid composition, Fourier transform infrared spectrometer (FTIR) and differential scanning calorimetry (DSC), in order to obtain the suitable prolamin extraction method and not affected by drying treatment. The research results showed that the yield and purity of ethanol-alkaline method were higher than those of acetic-acid method, and there were no significant differences in the molecular weight, amino acid composition, FTIR and DSC of prolamin of distiller's grains before and after drying. And this ensured the consistent quality of prolamin, so we thought that the ethanol-alkaline method was more suitable for the extraction of prolamin from distiller's grains. Overall, this research is beneficial to the subsequent application and development of prolamin, and can lay the foundation for the high value-added utilization of distiller's grains.
HOU Mengyuan
,
FAN Wenlai
,
XU Yan
. Extraction and characterization comparison of prolamin from wet and dried distiller's grains of Baijiu[J]. Food and Fermentation Industries, 2020
, 46(19)
: 99
-103
.
DOI: 10.13995/j.cnki.11-1802/ts.024122
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