The production technology of extracting collagen from tilapia skin by solid-state fermentation(SSF) with Bacillus subtilis was studied. The extraction rate of collagen was selected as the evaluation index, the fermentation conditions were optimized by single factor test and response surface methodology. The Fourier transform infrared spectra, ultraviolet spectra, sodium dodecyl sulfate poly-acrylamide gel electrophoresis (SDS-PAGE) and amino acid composition of tilapia skin SSF collagen and acid-soluble collagen (ASC) were analyzed and compared. The results showed that the optimum fermentation process parameters were cell age 20 h, water addition 11.7 mL, inoculum size 5.8%, fermentation temperature 34 °C, and fermentation time 48 h. Under such conditions, the extraction rate of SSF collagen (65.03%) was higher than that of ASC (57.26%), and both exhibited typical and similar collagen characteristics. This study demonstrated that SSF was a stable and reliable method for collagen production, providing a novel biotechnological process for deep processing of tilapia skin.
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