Fructose monolaurate was synthesized by Candida antarctica Lipase B(CALB) displayed on the cell surface of Pichia pastoris.The parameters affecting the two-step extraction of fructose monolaurate was studied.The fructose monolaurate could be obtained with a yeild of approximately 90% and with a purity of surpass 95% under optimal condition.The surface activities of fructose monolaurate were shown as following: HLB 5.775,CMC 0.25 mmol/L and critical surface tension γ 24.22 mN/m,indicating that it owns the good ability to change the surface activities of aqueous solution.Frutose monolaurate displayed better foaming power and foaming stability at low concentrations as well as similar emulsion power and emulsion stability comparing with that of the commercial surcrose esters.