Ligninolytic enzymes involved in lignin biodegradation are mainly produced by white-rot fungus.In order to enhance the ligninolytic enzymes activity of Pleurotus eryngii-Co007,the ligninolytic enzymes production curve and the influence on igninolytic enzymes production by fermentation conditions were studied.Pleurotus eryngii-Co007 was found to produce maximum amount of ligninolytic enzymes at the eighth day.The results of single factor tests were listed as follows: the optimum fermentation parameters were 10%~15%(v/v) inoculation volume,25~27℃ and pH 5.0~6.0,which the most significant factor was pH;maltose and organic nitrogen were propitious to strengthen the expression of ligninolytic enzyme.However,as far as the increase of the ligninolytic enzymes expression and production costs were concerned,the compositions of optimal fermentation medium were glucose 3%,yeast extract 0.2%,beef extract 0.2%;the effective inducers were straw,Cu2+ and Tween 80.