In the paper,the synthesis of phytosterol laurate in non-aqueous phase with lipase was studied.The optimal conditions were also obtained by single-factor and orthogonal experiments.The results showed that the yield of esterification reached the maximum(94.62%),when under the conditions of molar ratio of lauric acid to phytosterol 3∶1,adding amount of organic solvent 6mL/g,reaction temperature 45℃,lipase 11%,and reaction time 48 hrs.After purification,the content of phytesterol ester reached 98.2%.The structure of phytosterol laurate was confirmed with TLC,FT-IR and MS.Lipase can catalyze the synthesis of phytosterol esters in non-aqueous phase high efficiency.