We isolated a new strain-Monascus,from Fen Liquor daqu to produce high yield esters.We used Orthogonal optimization method to optimize the culture medium and culture conditions to obtain maximum esterases activity.The results showed that the optimal medium is bran and water at the ratio of 1∶1.The results also presented that the optimum fermentation conditions as follows: Monascus inoculation is 1%,the fermentation temperature is 30 ℃,the fermentation time is 4d.Based on these findings,we prepared esterases by Monascus.Meanwhile,we utilized the response surface methodology to determine the optimal conditions for catalytic reaction of ethyl lactate.The results showed that the molar ratio of ethanol and lactic acid was 6,the enzyme dosage was 1.5%,the reaction time was 60h,and the reaction temperature was 30 ℃.The yield of ethyl lactate can reach 3.38 g / L.This result can provide some experimental basis to develop the flavoring liquor ethyl lactate.