An efficient microwave-assisted extraction(MAE) technique was developed to extract antioxidants from Enokitake.The operating parameters were optimized using orthogonal experiment.Scavenging effect of Flammulina extract on DPPH radical was studied,The optimum extraction conditions were as follows: extraction temperature of 75℃,extraction time of 23 min,and solution-solid ratio of 6(g/mL),respectively.The antioxidant activities of the extracts were analyzed,and the results showed that the extracts presented strong ability of radical-scavenging properties against the DPPH and strong reducing power.It also inhibited the peroxidantion of chicken oil.