Food and Fermentation Industries

Research on the Property of an Oil-degrading Bacteria

  • Qi Xiang-jun ,
  • Xu Ting-ting ,
  • Zhang Wen ,
  • Ran Deng-yuan
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Online published: 2011-02-25

Abstract

In order to select a high-efficient oil-degrading bacteria,here an oil-degrading bacteria was selected from the lampblack exit of the restaurant,which was named QZX-A.Then,it was identified using the methods such as morphological,physiological and biochemical identification and 16S rRNA.At the same time,its growth performance was anaylzed by the turbidimetric and weighing methods.Furthmore,its ability to produce rhamnolipid was discussed by the method oil-spreading and anthrone-sulfuric acid.Results: On the basis of morphological,physiological and biochemical evidence,strain QZX-A had the characteristics of Pseudomonas aeruginosa bacteria.Phylogenetic analysis based on 16S rRNA gene sequences revealed that QZX-A belonged to a phyletic sub-lineage within the family Pseudomonas,with ≥98% 16S rRNA gene sequence similarity to the described species.In addition,strain QZX-A had a similar growth curve in beef extract peptone and fermentation medium,but the growth rate of the former was bigger than the latter.The result of TLC showed that the metabolic products of strain QZX-A belonged to glycolipid compounds.The oil-spreading round was up to 11cm,and its productivity was 567.75 mg /L.In sum,since strain QZX-A was genetically stable,it had some value of development in the degradation of oil and in the production of rhamnolipid.

Cite this article

Qi Xiang-jun , Xu Ting-ting , Zhang Wen , Ran Deng-yuan . Research on the Property of an Oil-degrading Bacteria[J]. Food and Fermentation Industries, 2011 , 37(02) : 99 -102 . DOI: 10.13995/j.cnki.11-1802/ts.2011.02.004

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