The production of mannitol by Leuconostoc mesenteroides CGMCC 1.10327 using sucrose(2%,m/v) as the carbon source was investigated in batch culture fermentation.To optimize the production of mannitol,the effects of glucose(5 g/L),three salt nutrients(K2HPO4,NaAC,and ammonium citrate),various initial culture pHs,and 0.2% CaCO3 on the production of mannitol were investigated,respectively.The results showed that glucose contributed to improve the yield of mannitol.Three salt nutrients(K2HPO4,NaAC,and ammonium citrate) had significant effects on the production of mannitol,and the highest yield of mannitol was obtained when three salt nutrients were 2 g/L,5.0 g/L,and 2 g/L,respectively.The highest yield of mannitol was obtained at initial pH 6.0.Moreover,the yield of mannitol was significantly decreased by adding CaCO3.