In order to study the changes of collagen from sea cucumber body wall during heating process,the AFM method was adopted to investigate the influence of sample concentration,solvents,heating processing temperature and heating time on the aggregation states of PSC from sea cucumber body wall.The results showed that in acetate buffer(pH 2.7),PBS buffer(pH 7.2) and ultra-pure water solution,collagen self-assembled gradually as the concentration increased and net-work of random coils or schistose structure were formed eventually.But the concentration of PSC needed to start self-assemble was different in the solutions mentioned above.The collagen self-assembled gradually to net-work as the heating temperature rised or the heating time prolonged.But,when the temperature was higher than 100 ℃ or the time was longer than 2 h,its crosslinking degree would decrease and the net-work structure disappeared gradually.