According to the 16S rDNA and hip O gene sequences of C.jejuni in GenBank,two pairs of specific primers were designed and used in multiplex PCR for detection of C.jejuni in animal origin food.Multiplex PCR was developed and applied to the sample testing.The results indicated that two specific fragments(699bp and 366bp) were detected after amplification of the DNA template of C.jejuni,while other bacteria strains(11 species tested) were not detected.Meanwhile,the 16S rDNA and hip O gene sequence of C.jejuni ATCC33560 exhibited a high similarity with those of some strains of C.jejuni presented in GenBank.The total assay could be completed in 27h with a detection limit of 2.4~16CFU/mL.The chicken,pork,beef and milk from Ya'an markets in Sichuan province were detected by the multiplex PCR,and 38.0%,28.3%,17.1% and 8.6% of them were found respectively to be positive for C.jejuni.Multiplex PCR assay was specific,sensitive and time-saving,which provided reference for detection of C.jejuni in animal origin food.